The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n=7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n=7) it was left till day 10, when PGF2a was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing. A higher (P<0.05) number of corpora lutea (8.3 vs. 5.7) and embryo/flushing/buffalo (2.3 vs. 1.3) were recorded in group B vs. group A if responsive buffaloes are considered (n=12) and the number of corpora lutea was highly correlated with the number of embryos (r=0.65; P<0.05). In conclusion, progesterone supplementation during the first 2 days of the superovulation treatment seems to enhance the recovery rate in buffalo species. A high ovulation rate, associated with a high number of corpora lutea, can represent a parameter for estimating embryo recovery.
Progesterone supplementation during multiple ovulation treatment in buffalo species (Bubalus bubalis) / Neglia, Gianluca; Gasparrini, Bianca; Vecchio, Domenico; Rubessa, M.; DI PALO, Rossella; Zicarelli, Luigi; Campanile, Giuseppe. - In: TROPICAL ANIMAL HEALTH AND PRODUCTION. - ISSN 0049-4747. - STAMPA. - 42:(2010), pp. 1243-1247. [10.1007/s11250-010-9556-8]
Progesterone supplementation during multiple ovulation treatment in buffalo species (Bubalus bubalis).
NEGLIA, GIANLUCA;GASPARRINI, BIANCA;VECCHIO, DOMENICO;DI PALO, ROSSELLA;ZICARELLI, LUIGI;CAMPANILE, GIUSEPPE
2010
Abstract
The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n=7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n=7) it was left till day 10, when PGF2a was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing. A higher (P<0.05) number of corpora lutea (8.3 vs. 5.7) and embryo/flushing/buffalo (2.3 vs. 1.3) were recorded in group B vs. group A if responsive buffaloes are considered (n=12) and the number of corpora lutea was highly correlated with the number of embryos (r=0.65; P<0.05). In conclusion, progesterone supplementation during the first 2 days of the superovulation treatment seems to enhance the recovery rate in buffalo species. A high ovulation rate, associated with a high number of corpora lutea, can represent a parameter for estimating embryo recovery.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.