BACKGROUND: IGF-1 is a potent mitogen for vascular smooth muscle cells, but exerts protective effects on endothelial cells that may trigger antiatherogenic mechanisms. OBJECTIVES: This study was designed to test the hypothesis that an IGF-1 excess following arterial injury prevents neointima formation and vascular stenosis. METHODS: Rats were subjected to carotid balloon injury and treated with IGF-1 (1.2 mg kg(-1) per die) or saline for 10 days. RESULTS: In IGF-1 treated animals, high tissue levels of eNOS, Akt and its phosphorylated form were found, confirming activation of IGF-1-dependent signaling pathways. IGF-1 markedly reduced neointima formation and post-injury arterial stenosis. IGF-1 exerted proliferative and anti-apoptotic effects in the media of injured carotids, but inhibited mitotic activity and induced apoptosis in the neointima. Furthermore, IGF-1 stimulated mobilization of progenitor endothelial cells and re-endothelialization of the injured arteries. L-NAME administration inhibited IGF-1 vasculoprotective effects. CONCLUSIONS: IGF-1 attenuates post-injury carotid stenosis by exerting differential effects in the neointima and tunica media with regard to the key components of the response to injury. The data point to a novel role of IGF-1 as a potent vasculoprotective factor.

Insulin-like growth factor-1 protects from vascular stenosis and accelerates reendothelialization in a rat model of carotid artery injury / Cittadini, Antonio; Monti, MARIA GAIA; Castiello, M.; D'Arco, E.; Galasso, Gennaro; Sorriento, D.; Saldamarco, Lavinia; DE PAULIS, Amato; Napoli, Raffaele; Iaccarino, Guido; Sacca', Luigi. - In: JOURNAL OF THROMBOSIS AND HAEMOSTASIS. - ISSN 1538-7933. - ELETTRONICO. - 7:11(2009), pp. 1920-1928. [10.1111/j.1538-7836.2009.03607.x]

Insulin-like growth factor-1 protects from vascular stenosis and accelerates reendothelialization in a rat model of carotid artery injury.

CITTADINI, ANTONIO;MONTI, MARIA GAIA;GALASSO, GENNARO;Sorriento D.;SALDAMARCO, LAVINIA;DE PAULIS, AMATO;NAPOLI, RAFFAELE;IACCARINO, GUIDO;SACCA', LUIGI
2009

Abstract

BACKGROUND: IGF-1 is a potent mitogen for vascular smooth muscle cells, but exerts protective effects on endothelial cells that may trigger antiatherogenic mechanisms. OBJECTIVES: This study was designed to test the hypothesis that an IGF-1 excess following arterial injury prevents neointima formation and vascular stenosis. METHODS: Rats were subjected to carotid balloon injury and treated with IGF-1 (1.2 mg kg(-1) per die) or saline for 10 days. RESULTS: In IGF-1 treated animals, high tissue levels of eNOS, Akt and its phosphorylated form were found, confirming activation of IGF-1-dependent signaling pathways. IGF-1 markedly reduced neointima formation and post-injury arterial stenosis. IGF-1 exerted proliferative and anti-apoptotic effects in the media of injured carotids, but inhibited mitotic activity and induced apoptosis in the neointima. Furthermore, IGF-1 stimulated mobilization of progenitor endothelial cells and re-endothelialization of the injured arteries. L-NAME administration inhibited IGF-1 vasculoprotective effects. CONCLUSIONS: IGF-1 attenuates post-injury carotid stenosis by exerting differential effects in the neointima and tunica media with regard to the key components of the response to injury. The data point to a novel role of IGF-1 as a potent vasculoprotective factor.
2009
Insulin-like growth factor-1 protects from vascular stenosis and accelerates reendothelialization in a rat model of carotid artery injury / Cittadini, Antonio; Monti, MARIA GAIA; Castiello, M.; D'Arco, E.; Galasso, Gennaro; Sorriento, D.; Saldamarco, Lavinia; DE PAULIS, Amato; Napoli, Raffaele; Iaccarino, Guido; Sacca', Luigi. - In: JOURNAL OF THROMBOSIS AND HAEMOSTASIS. - ISSN 1538-7933. - ELETTRONICO. - 7:11(2009), pp. 1920-1928. [10.1111/j.1538-7836.2009.03607.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/357576
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