Computer program for post-translational modification site assignment in proteins using fast atom bombardment mass spectral data

A computer program allowing post-translational modification sites assignment in proteins has been developed. The program has been constructed to elaborate data obtained from fast atom bombardment mass spectrometric mapping of polypeptides. The mass values of peptide(s) which cannot be assigned into the protein primary structure are elaborated by the program, which allows identification of the modified peptide(s) as well as the nature of the modifying group(s). This procedure has been applied to different kinds of post-translational events using three proteins as a model.