In the present study we investigated whether apoptosis and phagocytosis are regulated by nuclear factor (NF)-kB in a model of chronic inflammation. The subcutaneous implant of carrageenin-soaked sponges elicited an inflammatory response, characterized by a time-related increase of leukocyte infiltration into the sponge and tissue formation, which was inhibited by simultaneous injection of wild-type oligodeoxynucleotide decoy to NF-kB. Molecular and morphological analysis performed on infiltrated cells demonstrated: 1) an inhibition of NF-kB/DNA binding activity; 2) an increase of polymorphonuclear leukocyte apoptosis correlated either to an increase of p53 or Bax and decrease of Bcl-2 protein expression; and 3) an increase of phagocytosis of apoptotic polymorphonuclear leukocytes by macrophages associated with an increase of transforming growth factor-beta and decrease of tumor necrosis factor- alpha as well as nitrite/ nitrate production. Our results, showing that blockade of NF-kB by oligodeoxynucleotide decoy increases inflammatory cell apoptosis and phagocytosis, may contribute to lead to new insights into the mechanisms governing the inflammatory process. (Am J Pathol 2004, 165:115–126)

Nuclear factor-kappaB regulates inflammatory cell apoptosis and phagocytosis in rat carrageenin-sponge implant model / Maiuri, MARIA CHIARA; Tajana, G.; Iuvone, Teresa; De Stefano, D.; Mele, G.; Ribecco, M. T.; Cinelli, Mariapia; Romano, MARIA FIAMMETTA; Turco, M. C.; Carnuccio, Rosa. - In: THE AMERICAN JOURNAL OF PATHOLOGY. - ISSN 0002-9440. - STAMPA. - 165:1(2004), pp. 115-126. [10.1016/S0002-9440(10)63280-4]

Nuclear factor-kappaB regulates inflammatory cell apoptosis and phagocytosis in rat carrageenin-sponge implant model.

MAIURI, MARIA CHIARA;IUVONE, TERESA;CINELLI, MARIAPIA;ROMANO, MARIA FIAMMETTA;CARNUCCIO, ROSA
2004

Abstract

In the present study we investigated whether apoptosis and phagocytosis are regulated by nuclear factor (NF)-kB in a model of chronic inflammation. The subcutaneous implant of carrageenin-soaked sponges elicited an inflammatory response, characterized by a time-related increase of leukocyte infiltration into the sponge and tissue formation, which was inhibited by simultaneous injection of wild-type oligodeoxynucleotide decoy to NF-kB. Molecular and morphological analysis performed on infiltrated cells demonstrated: 1) an inhibition of NF-kB/DNA binding activity; 2) an increase of polymorphonuclear leukocyte apoptosis correlated either to an increase of p53 or Bax and decrease of Bcl-2 protein expression; and 3) an increase of phagocytosis of apoptotic polymorphonuclear leukocytes by macrophages associated with an increase of transforming growth factor-beta and decrease of tumor necrosis factor- alpha as well as nitrite/ nitrate production. Our results, showing that blockade of NF-kB by oligodeoxynucleotide decoy increases inflammatory cell apoptosis and phagocytosis, may contribute to lead to new insights into the mechanisms governing the inflammatory process. (Am J Pathol 2004, 165:115–126)
2004
Nuclear factor-kappaB regulates inflammatory cell apoptosis and phagocytosis in rat carrageenin-sponge implant model / Maiuri, MARIA CHIARA; Tajana, G.; Iuvone, Teresa; De Stefano, D.; Mele, G.; Ribecco, M. T.; Cinelli, Mariapia; Romano, MARIA FIAMMETTA; Turco, M. C.; Carnuccio, Rosa. - In: THE AMERICAN JOURNAL OF PATHOLOGY. - ISSN 0002-9440. - STAMPA. - 165:1(2004), pp. 115-126. [10.1016/S0002-9440(10)63280-4]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/343712
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