The double staining Trypan blue/Giemsa has been used to evaluate the semen of two stallions (A and B) bred in the same farm. In order to compare the fertility rate of the last mating season with the results of this staining technique, semen samples were collected at 10 days intervals and stained according to Kovács and Foote (1992). Sperm cells were then classified as: live with intact acrosome (VCA), live with acrosome reacted (VAR), dead with intact acrosome (MCA), dead with acrosome reacted (MAR), dead with damaged acrosome (MAD), only sperm head (ST). Mating data and pregnancy rate at 40 days of gestation were collected in order to evaluate the first cycle pregnancy rate and the seasonal pregnancy rate. Differences of pregnancy rate between stallions were analyzed by Chi Square test. Differences of sperm cells percentage in the staining classes were analyzed by ANOVA, after the angular transformations. Seasonal pregnancy rate were different between stallions (85,7% vs 52,9% in A vs B; P<0,05). According to the fertility data, higher percentages of live sperms (P< 0.01), VCA (P< 0.01), MAR (P<0.05) and lower percentages of VAR (P<0.05), MCA (P<0.01) and MAD (P<0.01) were found in A vs B stallion
Evaluation of stallion semen by trypan blue /giemsa staining and related fertility / Santese, G.; Boccia, L.; Spadetta, M.; Attanasio, Laura; DE ROSA, A.; Gasparrini, Bianca; Campanile, Giuseppe; DI PALO, Rossella. - STAMPA. - 1:(2006), pp. 41-46. (Intervento presentato al convegno 8th National Meeting “New findings in equine practices”, Italian Society for Equine Practitioners tenutosi a Campobasso -Italy nel June, 23/ 2006).
Evaluation of stallion semen by trypan blue /giemsa staining and related fertility.
ATTANASIO, LAURA;GASPARRINI, BIANCA;CAMPANILE, GIUSEPPE;DI PALO, ROSSELLA
2006
Abstract
The double staining Trypan blue/Giemsa has been used to evaluate the semen of two stallions (A and B) bred in the same farm. In order to compare the fertility rate of the last mating season with the results of this staining technique, semen samples were collected at 10 days intervals and stained according to Kovács and Foote (1992). Sperm cells were then classified as: live with intact acrosome (VCA), live with acrosome reacted (VAR), dead with intact acrosome (MCA), dead with acrosome reacted (MAR), dead with damaged acrosome (MAD), only sperm head (ST). Mating data and pregnancy rate at 40 days of gestation were collected in order to evaluate the first cycle pregnancy rate and the seasonal pregnancy rate. Differences of pregnancy rate between stallions were analyzed by Chi Square test. Differences of sperm cells percentage in the staining classes were analyzed by ANOVA, after the angular transformations. Seasonal pregnancy rate were different between stallions (85,7% vs 52,9% in A vs B; P<0,05). According to the fertility data, higher percentages of live sperms (P< 0.01), VCA (P< 0.01), MAR (P<0.05) and lower percentages of VAR (P<0.05), MCA (P<0.01) and MAD (P<0.01) were found in A vs B stallionI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
