In this paper an analytical method for the extraction and the determination by HPLC-UV of flavone (2-phenyl-4-H-1-benzopyran-4-one) in human serum has been developed. This analyte may have some effects on drug metabolism, interacting with microsomial hepatic enzymes. The proposed method is rapid and simple, with high recoveries (98%), good sensitivity (LOD 7,5 g/mL) and reproducibility (C.V. 8%) and could be utilized for routine epidemiological analysis. Preliminary pharmacokinetics studies showed that flavone hits the maximum blood level into two hours.
HPLC determination of flavone in human serum / Morrica, Patrizia; Marra, Maurizio; Seccia, Serenella; Ventriglia, Mariacarla; Moro, CARLA ORNELLA. - In: AMERICAN LABORATORY. - ISSN 0044-7749. - STAMPA. - 29:(1997), pp. 36-39.
HPLC determination of flavone in human serum
MORRICA, PATRIZIA;MARRA, MAURIZIO;SECCIA, SERENELLA;VENTRIGLIA, MARIACARLA;MORO, CARLA ORNELLA
1997
Abstract
In this paper an analytical method for the extraction and the determination by HPLC-UV of flavone (2-phenyl-4-H-1-benzopyran-4-one) in human serum has been developed. This analyte may have some effects on drug metabolism, interacting with microsomial hepatic enzymes. The proposed method is rapid and simple, with high recoveries (98%), good sensitivity (LOD 7,5 g/mL) and reproducibility (C.V. 8%) and could be utilized for routine epidemiological analysis. Preliminary pharmacokinetics studies showed that flavone hits the maximum blood level into two hours.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
