The aim is to determine the monocyte count performance of the Bayer Diagnostics ADVIA 120 and Coulter LH 750 automated haematology analysers and the results obtained by these two instruments were compared with those provided by Becton Dickinson FACScan flow cytometer using the combination of CD45/CD14 MoAb. Linearity and imprecision were also evaluated. The linearity of both instruments was good. Coulter LH 750 showed better precision (4.3%) than ADVIA 120 (9.0%) both within and between batch. A significant correlation (r ¼ 0.973) was found between the LH 750 and the flow cytometry method, while a modest one was observed between the latter and the ADVIA 120 (r ¼ 0.880). When comparing the percentage of monocytes by means of one-way anova and Tukey test, it was found that the LH 750 provided the closest results in comparison with flow cytometry, with no statistical difference between the means (mean difference MO% ¼ 0.6); however the difference was statistically different between the ADVIA 120 and flow cytometry (mean difference MO% ¼ )4.06). These data were confirmed by Altman–Bland and Deming regression analyses.

Evaluation of the monocyte counting by two automated haematology analysers compared with flow cytometry.

GRIMALDI, ERNESTO;CARANDENTE GIARRUSSO, PATRIZIA;SCOPACASA, FRANCESCO UMBERTO VITTOR;ROMANO, MARIA FIAMMETTA;PELLEGRINO, MARIA;BISOGNI, RITA;DE CATERINA, MAURIZIO
2005

Abstract

The aim is to determine the monocyte count performance of the Bayer Diagnostics ADVIA 120 and Coulter LH 750 automated haematology analysers and the results obtained by these two instruments were compared with those provided by Becton Dickinson FACScan flow cytometer using the combination of CD45/CD14 MoAb. Linearity and imprecision were also evaluated. The linearity of both instruments was good. Coulter LH 750 showed better precision (4.3%) than ADVIA 120 (9.0%) both within and between batch. A significant correlation (r ¼ 0.973) was found between the LH 750 and the flow cytometry method, while a modest one was observed between the latter and the ADVIA 120 (r ¼ 0.880). When comparing the percentage of monocytes by means of one-way anova and Tukey test, it was found that the LH 750 provided the closest results in comparison with flow cytometry, with no statistical difference between the means (mean difference MO% ¼ 0.6); however the difference was statistically different between the ADVIA 120 and flow cytometry (mean difference MO% ¼ )4.06). These data were confirmed by Altman–Bland and Deming regression analyses.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/337166
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 32
  • ???jsp.display-item.citation.isi??? 38
social impact