GnRH administration in buffalo species caused an increase of progesterone 96 hours after treatment. This aspect may result useful in order to increase progesterone blood levels and reduce the phenomenon of embryo mortality in buffalo. The aim of this study was to verify the pituitary response to a GnRH administration on day 6 post-insemination in buffalo. The trial was carried out in January on 21 pluriparous Italian Mediterranean Buffaloes (DIM=118±50 days). The animals were synchronized by using the Ovsynch-TAI Program and inseminated twice, 16 and 40 hours after the last administration of GnRH, with frozen semen. On day 6 post-insemination, buffaloes were treated with 12 μg of Buserelin acetate. Blood samples were collected just before GnRH administration (time 0 – T0) and 30 minutes (time 1 – T1), 3 hours (time 3 – T3) and 24 hours (time 24 – T24) after administration, in order to determine LH, FSH and E2 levels by using a double antibody radioimmuno assay (RIA) on plasma. Pregnancy diagnosis was performed 40 days post-insemination by ultrasound. The differences between the mean levels of LH, FSH and E2 after Buserelin acetate administration were analysed by t Student test. The administration of 12 mcg of Buserelin acetate significantly (P<0.01) increased LH and FSH blood levels after 30 minutes (T1) and after 3 hours (T3), lowering to the initial values after 24 hours (T24). Similarly to these hormones, the estradiol significantly (P<0.01) increased after 30 minutes (T1), decreasing to the basal levels after 3 hours (T3). The use of GnRH or some analogues determines in buffalo as in cattle a pituitary response after only 30 minutes from the administration. Similar results have been recorded from other authors, who reported a P4 increase after 96 hours from the treatment. This increase was more evident after 10 days. Therefore the use of GnRH or its analogous represents a good tool in order to increase P4 levels in buffalo species. This aspect may result useful in order to improve the uterine environment and favourite embryo implantation.

Effect of GnRH administration on progesterone blood levels in buffalo.

NEGLIA, GIANLUCA;BOCCIA, LUCIA;
2005

Abstract

GnRH administration in buffalo species caused an increase of progesterone 96 hours after treatment. This aspect may result useful in order to increase progesterone blood levels and reduce the phenomenon of embryo mortality in buffalo. The aim of this study was to verify the pituitary response to a GnRH administration on day 6 post-insemination in buffalo. The trial was carried out in January on 21 pluriparous Italian Mediterranean Buffaloes (DIM=118±50 days). The animals were synchronized by using the Ovsynch-TAI Program and inseminated twice, 16 and 40 hours after the last administration of GnRH, with frozen semen. On day 6 post-insemination, buffaloes were treated with 12 μg of Buserelin acetate. Blood samples were collected just before GnRH administration (time 0 – T0) and 30 minutes (time 1 – T1), 3 hours (time 3 – T3) and 24 hours (time 24 – T24) after administration, in order to determine LH, FSH and E2 levels by using a double antibody radioimmuno assay (RIA) on plasma. Pregnancy diagnosis was performed 40 days post-insemination by ultrasound. The differences between the mean levels of LH, FSH and E2 after Buserelin acetate administration were analysed by t Student test. The administration of 12 mcg of Buserelin acetate significantly (P<0.01) increased LH and FSH blood levels after 30 minutes (T1) and after 3 hours (T3), lowering to the initial values after 24 hours (T24). Similarly to these hormones, the estradiol significantly (P<0.01) increased after 30 minutes (T1), decreasing to the basal levels after 3 hours (T3). The use of GnRH or some analogues determines in buffalo as in cattle a pituitary response after only 30 minutes from the administration. Similar results have been recorded from other authors, who reported a P4 increase after 96 hours from the treatment. This increase was more evident after 10 days. Therefore the use of GnRH or its analogous represents a good tool in order to increase P4 levels in buffalo species. This aspect may result useful in order to improve the uterine environment and favourite embryo implantation.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/336198
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