A comparative chromosome analysis was performed on day-7 in vitro produced buffalo embryos, grown either in SOF or KSOM media. Cumulus Oocyte Complexes from abattoir derived ovaries were in vitro matured and fertilized. On Day 1 (Day 0= IVF Day), presumptive zygotes were allocated randomly in SOF or KSOM media until day 7, when embryo output was evaluated. Preliminary cytogenetic analysis indicate that SOF or KSOM embryos show similar mean number of cells ((69.6 vs 68.4) and similar mitotic index (5.9 vs. 7.5). A higher incidence of chromosome abnormalities were found in SOF comared to KSOM embryos (23.7% vs 10.3%).
Comparative chromosome analysis of day-7 in vitro produced river buffalo (Bubalus bubalis) embryos grown in SOF and KSOM media / Coppola, G. F.; Neglia, Gianluca; Gasparrini, Bianca; Coppola, G.; CARACCIOLO DI BRIENZA, V.; Zicarelli, Luigi; Enne, G.; DI BERARDINO, D.. - STAMPA. - (2002), pp. 52-52. (Intervento presentato al convegno 15th European Colloquium on Cytogenetics of Domestic Animals and Gene Mapping tenutosi a Sorrento, Naples, Italy nel June 2 - 4, 2002).
Comparative chromosome analysis of day-7 in vitro produced river buffalo (Bubalus bubalis) embryos grown in SOF and KSOM media.
NEGLIA, GIANLUCA;GASPARRINI, BIANCA;ZICARELLI, LUIGI;
2002
Abstract
A comparative chromosome analysis was performed on day-7 in vitro produced buffalo embryos, grown either in SOF or KSOM media. Cumulus Oocyte Complexes from abattoir derived ovaries were in vitro matured and fertilized. On Day 1 (Day 0= IVF Day), presumptive zygotes were allocated randomly in SOF or KSOM media until day 7, when embryo output was evaluated. Preliminary cytogenetic analysis indicate that SOF or KSOM embryos show similar mean number of cells ((69.6 vs 68.4) and similar mitotic index (5.9 vs. 7.5). A higher incidence of chromosome abnormalities were found in SOF comared to KSOM embryos (23.7% vs 10.3%).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
