Oligonucleotides conjugated at both 30 and 50-ends with glucose residues, 4,6-linked through a phosphodiester bridge, have been synthesized by sequential addition of a 6-O-DMT-glucose-4-phosphoramidite building block following a standard automated ODN assembly procedure. Two 30,50-bis-glycoconjugated 18-mers, designed for antisense experiments, have been prepared and their hybridization properties with a complementary DNA fragment evaluated by UV thermal analysis
SOLID PHASE SYNTHESIS OF OLIGONUCLEOTIDES TETHERED TO OLIGO-GLUCOSE PHOSPHATE TAILS
DE NAPOLI, LORENZO;DI FABIO, GIOVANNI;IADONISI, ALFONSO;MONTESARCHIO, DANIELA;PICCIALLI, GENNARO
2002
Abstract
Oligonucleotides conjugated at both 30 and 50-ends with glucose residues, 4,6-linked through a phosphodiester bridge, have been synthesized by sequential addition of a 6-O-DMT-glucose-4-phosphoramidite building block following a standard automated ODN assembly procedure. Two 30,50-bis-glycoconjugated 18-mers, designed for antisense experiments, have been prepared and their hybridization properties with a complementary DNA fragment evaluated by UV thermal analysisFile in questo prodotto:
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