A solid phase oligonucleotide (ON) synthesis on porous silicon (PSi) chip is presented. The prepared Si- OH surface were analyzed by FT-IR and the OH functions were quantified by reaction with 3'- phosphoramidite nucleotide building block. Short ONs were synthesized on the chip surface and the coupling yields evaluated.
Oligonucleotides direct synthesis on porous silicon chip / DE STEFANO, L.; DE TOMMASI, E.; Rea, E.; Rotiroti, Lucia; Giangrande, L.; Oliviero, Giorgia; Borbone, Nicola; Galeone, Aldo; Piccialli, Gennaro. - In: NUCLEIC ACIDS SYMPOSIUM SERIES. - ISSN 0261-3166. - 52:(2008), pp. 721-722. [10.1093/nass/nrn364]
Oligonucleotides direct synthesis on porous silicon chip
ROTIROTI, LUCIA;OLIVIERO, GIORGIA;BORBONE, NICOLA;GALEONE, ALDO;PICCIALLI, GENNARO
2008
Abstract
A solid phase oligonucleotide (ON) synthesis on porous silicon (PSi) chip is presented. The prepared Si- OH surface were analyzed by FT-IR and the OH functions were quantified by reaction with 3'- phosphoramidite nucleotide building block. Short ONs were synthesized on the chip surface and the coupling yields evaluated.| File | Dimensione | Formato | |
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2008 NucAcSymSer Oligo.pdf
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