In this work we investigated the interaction between the short ac4a C-rich peptide nucleic acid (PNA) probe and two intramolecular G-quadruplex targets having the same G-tetrad core, but different folding topologies. The T(G4T)3G4T and the recently reported tetra-end-linked-(TG4T)4 G-rich oligonucleotides (GROs) were chosen and synthesized for this study. UV, CD, and MS experiments revealed the formation of novel 1:1 G-quadruplex-PNA complexes besides the expected DNA-PNA heteroduplexes
A short C-rich PNA fragment capable to form novel G-quadruplex-PNA complexes / Amato, Jussara; Gabelica, V.; Borbone, Nicola; Rosu, F.; DE PAUW, E.; Oliviero, Giorgia; Piccialli, Gennaro; Mayol, Luciano. - In: NUCLEIC ACIDS SYMPOSIUM SERIES. - ISSN 0261-3166. - 52:(2008), pp. 167-168. [10.1093/nass/nrn085]
A short C-rich PNA fragment capable to form novel G-quadruplex-PNA complexes.
AMATO, JUSSARA;BORBONE, NICOLA;OLIVIERO, GIORGIA;PICCIALLI, GENNARO;MAYOL, LUCIANO
2008
Abstract
In this work we investigated the interaction between the short ac4a C-rich peptide nucleic acid (PNA) probe and two intramolecular G-quadruplex targets having the same G-tetrad core, but different folding topologies. The T(G4T)3G4T and the recently reported tetra-end-linked-(TG4T)4 G-rich oligonucleotides (GROs) were chosen and synthesized for this study. UV, CD, and MS experiments revealed the formation of novel 1:1 G-quadruplex-PNA complexes besides the expected DNA-PNA heteroduplexesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
