The enzyme S-adenosylmethionine-DNA (cytosine-5)-methyltransferase has been identified, first time for invertebrates, in embryos of the marine polychaete annelid worm Chaetopterus variopedatus. The molecule has been isolated from embryos at 15 h of development. It is a single peptide of about 200 kDa molecular weight, cross-reacting with antibodies against sea urchin DNA methyltransferase. The enzymatic properties of the molecule are similar to those of Dnmt1 methyltransferases isolated from other organisms, but with the peculiarity to be unable to make 'de novo' methylation on double stranded DNA

Characterization of a new variant DNA (cytosine-5)-methyltransferase unable to methylate double stranded DNA isolated from the marine annelid worm Chaetopterus variopedatus / DEL GAUDIO, Rosanna; DI GIAIMO, Rossella; N., Potenza; M., Branno; Aniello, Francesco; Geraci, Giuseppe. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 460:2(1999), pp. 380-384. [10.1016/S0014-5793(99)01379-4]

Characterization of a new variant DNA (cytosine-5)-methyltransferase unable to methylate double stranded DNA isolated from the marine annelid worm Chaetopterus variopedatus

DEL GAUDIO, ROSANNA;DI GIAIMO, ROSSELLA;ANIELLO, FRANCESCO;GERACI, GIUSEPPE
1999

Abstract

The enzyme S-adenosylmethionine-DNA (cytosine-5)-methyltransferase has been identified, first time for invertebrates, in embryos of the marine polychaete annelid worm Chaetopterus variopedatus. The molecule has been isolated from embryos at 15 h of development. It is a single peptide of about 200 kDa molecular weight, cross-reacting with antibodies against sea urchin DNA methyltransferase. The enzymatic properties of the molecule are similar to those of Dnmt1 methyltransferases isolated from other organisms, but with the peculiarity to be unable to make 'de novo' methylation on double stranded DNA
1999
Characterization of a new variant DNA (cytosine-5)-methyltransferase unable to methylate double stranded DNA isolated from the marine annelid worm Chaetopterus variopedatus / DEL GAUDIO, Rosanna; DI GIAIMO, Rossella; N., Potenza; M., Branno; Aniello, Francesco; Geraci, Giuseppe. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 460:2(1999), pp. 380-384. [10.1016/S0014-5793(99)01379-4]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/316468
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