We report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of two heterologous antigens on the spore coat. Recombinant spores expressing tetanus toxin fragment C (TTFC) of Clostridium tetani or the B subunit of the heat-labile toxin of Escherichia coli (LTB) were used for oral dosing and shown to generate specific systemic and mucosal immune responses in a murine model. This report, expanding the previously described expression of TTFC on the spore surface by fusion to CotB [J Bacteriol 183 (2001) 6294] and its use for oral vaccination [Infect Immun 71 (2003) 2810] shows that different antigens can be successfully presented on the spore coat and supports the use of the spore as an efficient vehicle for mucosal immunisation.

Display of heterologous antigens on the Bacillus subtilis spore coat using CotC as a fusion partner / Mauriello, Em; DUC LE, H; Isticato, Rachele; Cangiano, Giuseppina; Hong, Ha; DE FELICE, Maurilio; Ricca, Ezio; Cutting, S. M.. - In: VACCINE. - ISSN 0264-410X. - STAMPA. - 22:9-10(2004), pp. 1177-1187. [10.1016/j.vaccine.2003.09.031]

Display of heterologous antigens on the Bacillus subtilis spore coat using CotC as a fusion partner.

ISTICATO, RACHELE;CANGIANO, GIUSEPPINA;DE FELICE, MAURILIO;RICCA, EZIO;
2004

Abstract

We report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of two heterologous antigens on the spore coat. Recombinant spores expressing tetanus toxin fragment C (TTFC) of Clostridium tetani or the B subunit of the heat-labile toxin of Escherichia coli (LTB) were used for oral dosing and shown to generate specific systemic and mucosal immune responses in a murine model. This report, expanding the previously described expression of TTFC on the spore surface by fusion to CotB [J Bacteriol 183 (2001) 6294] and its use for oral vaccination [Infect Immun 71 (2003) 2810] shows that different antigens can be successfully presented on the spore coat and supports the use of the spore as an efficient vehicle for mucosal immunisation.
2004
Display of heterologous antigens on the Bacillus subtilis spore coat using CotC as a fusion partner / Mauriello, Em; DUC LE, H; Isticato, Rachele; Cangiano, Giuseppina; Hong, Ha; DE FELICE, Maurilio; Ricca, Ezio; Cutting, S. M.. - In: VACCINE. - ISSN 0264-410X. - STAMPA. - 22:9-10(2004), pp. 1177-1187. [10.1016/j.vaccine.2003.09.031]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/315062
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