A polymorphic non-coding region of chloroplast DNA (cpDNA) was amplified by the polymerase chain reaction (PCR) from herbarium specimens of Pinus brutia and P. halepensis. The samples belong to the historical collection of Michele Tenore. Several discordances and inconsistencies in taxonomic attribution are present for historical specimens of P. brutia. Moreover, there are inaccuracies as to their geographical distribution. In this work, we demonstrate the potential use of molecular methods of amplifying DNA from historical samples to resolve the identification of herbarium specimens.
PCR amplification of Michele Tenore’s historical specimens and facility to utilize an alternative approach to resolve taxonomic problems / DE CASTRO, Olga; Menale, Bruno. - In: TAXON. - ISSN 0040-0262. - STAMPA. - 53:(2004), pp. 147-151.
PCR amplification of Michele Tenore’s historical specimens and facility to utilize an alternative approach to resolve taxonomic problems
DE CASTRO, OLGA;MENALE, BRUNO
2004
Abstract
A polymorphic non-coding region of chloroplast DNA (cpDNA) was amplified by the polymerase chain reaction (PCR) from herbarium specimens of Pinus brutia and P. halepensis. The samples belong to the historical collection of Michele Tenore. Several discordances and inconsistencies in taxonomic attribution are present for historical specimens of P. brutia. Moreover, there are inaccuracies as to their geographical distribution. In this work, we demonstrate the potential use of molecular methods of amplifying DNA from historical samples to resolve the identification of herbarium specimens.| File | Dimensione | Formato | |
|---|---|---|---|
|
8-Taxon04.pdf
non disponibili
Tipologia:
Documento in Pre-print
Licenza:
Accesso privato/ristretto
Dimensione
479.12 kB
Formato
Adobe PDF
|
479.12 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
