Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex.
Targeting DNA duplex with DNA-PNA chimeras? Physico-chemical characterization of a triplex DNA-PNA/DNA/DNA / Petraccone, Luigi; E., Erra; DE NAPOLI, Lorenzo; A., Messere; Montesarchio, Daniela; Piccialli, Gennaro; G., Barone; Giancola, Concetta. - In: BIOPOLYMERS. - ISSN 0006-3525. - STAMPA. - 73:4(2004), pp. 434-442. [10.1002/bip.10599]
Targeting DNA duplex with DNA-PNA chimeras? Physico-chemical characterization of a triplex DNA-PNA/DNA/DNA
PETRACCONE, LUIGI;DE NAPOLI, LORENZO;MONTESARCHIO, DANIELA;PICCIALLI, GENNARO;GIANCOLA, CONCETTA
2004
Abstract
Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
