The design of modified nucleic acid aptamers is improved by considering thermodynamics and kinetics of their association/dissociation processes. Locked Nucleic Acids (LNA) is a promising class of nucleic acid analogs. In this work the thermodynamic and kinetic properties of a LNA quadruplex formed by the TGGGT sequence, containing only conformationally restricted LNA residues, are reported and compared to those of 2'-OMe-RNA (O-RNA) and DNA quadruplexes. The thermodynamic analysis indicates that the sugar-modified quadruplexes (LNA and O-RNA) are stabilized by entropic effects. The kinetic analysis shows that LNA and O-RNA quadruplexes are characterized by a slower dissociation and a faster association with respect to DNA quadruplex. Interestingly, the LNA quadruplex formation process shows a second-order kinetics with respect to single strand concentration and has a negative activation energy. To explain these data, a mechanism for tetramer formation with two intermediate states was proposed.

Stabilità di una quadrupla elica di LNA / Petraccone, Luigi; Erra, Eva; Esposito, Veronica; Randazzo, Antonio; Mayol, Luciano; Barone, Guido; Giancola, Concetta. - STAMPA. - (2005), pp. 206-206. (Intervento presentato al convegno XXXIV Congresso Nazionale di Chimica Fisica tenutosi a Siena, Italy. nel 20-24 Giugno 2005).

Stabilità di una quadrupla elica di LNA

PETRACCONE, LUIGI;ERRA, EVA;ESPOSITO, VERONICA;RANDAZZO, ANTONIO;MAYOL, LUCIANO;BARONE, GUIDO;GIANCOLA, CONCETTA
2005

Abstract

The design of modified nucleic acid aptamers is improved by considering thermodynamics and kinetics of their association/dissociation processes. Locked Nucleic Acids (LNA) is a promising class of nucleic acid analogs. In this work the thermodynamic and kinetic properties of a LNA quadruplex formed by the TGGGT sequence, containing only conformationally restricted LNA residues, are reported and compared to those of 2'-OMe-RNA (O-RNA) and DNA quadruplexes. The thermodynamic analysis indicates that the sugar-modified quadruplexes (LNA and O-RNA) are stabilized by entropic effects. The kinetic analysis shows that LNA and O-RNA quadruplexes are characterized by a slower dissociation and a faster association with respect to DNA quadruplex. Interestingly, the LNA quadruplex formation process shows a second-order kinetics with respect to single strand concentration and has a negative activation energy. To explain these data, a mechanism for tetramer formation with two intermediate states was proposed.
2005
Stabilità di una quadrupla elica di LNA / Petraccone, Luigi; Erra, Eva; Esposito, Veronica; Randazzo, Antonio; Mayol, Luciano; Barone, Guido; Giancola, Concetta. - STAMPA. - (2005), pp. 206-206. (Intervento presentato al convegno XXXIV Congresso Nazionale di Chimica Fisica tenutosi a Siena, Italy. nel 20-24 Giugno 2005).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/311324
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