Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.
Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins / Amoroso, M. G.; Longobardo, Luigi; Capparelli, R.. - In: BIOTECHNOLOGY LETTERS. - ISSN 0141-5492. - ELETTRONICO. - 26:22(2004), pp. 1731-1737.
Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins
LONGOBARDO, LUIGI;R. Capparelli
2004
Abstract
Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.| File | Dimensione | Formato | |
|---|---|---|---|
|
Real Time RT-PCR and flow cytometry to investigate wheat kernel.pdf
non disponibili
Tipologia:
Documento in Post-print
Licenza:
Accesso privato/ristretto
Dimensione
104.89 kB
Formato
Adobe PDF
|
104.89 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
