The interaction between clupeine YI and various nucleotides in aq. soln. has been investigated with the aim of understanding the reason for the stronger affinity of purinic nucleotides towards the protein as compared with pyrimidinic ones. The study has been performed by 1H NMR spectrometry by adding increasing amts. of nucleotide to the protamine soln. The results obtained suggest that it is not the nature (purinic or pyrimidinic) of the nucleotide but its ability to give rise to a self-aggregation process that is crucial in the interaction with the protein. In fact, nucleotide complexation should provide a polyanionic matrix around which the protein can be strongly linked. This model was tested with different natural, synthetic, and modified nucleotides and with some dinucleotides.

The role of nucleotide self-aggregation on the interaction process between clupeine YI (a fish protamine) and mono- and dinucleotides / Andini, Salvatore; Ferrara, Luciano; B., Cocozziello; L., De Napoli; G., Piccialli; S., Barbato. - In: GAZZETTA CHIMICA ITALIANA. - ISSN 0016-5603. - STAMPA. - 119:5(1989), pp. 271-275.

The role of nucleotide self-aggregation on the interaction process between clupeine YI (a fish protamine) and mono- and dinucleotides.

ANDINI, SALVATORE;FERRARA, LUCIANO;
1989

Abstract

The interaction between clupeine YI and various nucleotides in aq. soln. has been investigated with the aim of understanding the reason for the stronger affinity of purinic nucleotides towards the protein as compared with pyrimidinic ones. The study has been performed by 1H NMR spectrometry by adding increasing amts. of nucleotide to the protamine soln. The results obtained suggest that it is not the nature (purinic or pyrimidinic) of the nucleotide but its ability to give rise to a self-aggregation process that is crucial in the interaction with the protein. In fact, nucleotide complexation should provide a polyanionic matrix around which the protein can be strongly linked. This model was tested with different natural, synthetic, and modified nucleotides and with some dinucleotides.
1989
The role of nucleotide self-aggregation on the interaction process between clupeine YI (a fish protamine) and mono- and dinucleotides / Andini, Salvatore; Ferrara, Luciano; B., Cocozziello; L., De Napoli; G., Piccialli; S., Barbato. - In: GAZZETTA CHIMICA ITALIANA. - ISSN 0016-5603. - STAMPA. - 119:5(1989), pp. 271-275.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/307250
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