Mutagen activity evaluation of enrofloxacin and ciprofloxacin on murine erythroleukemia cells. Valutazione dell’attività mutagena dell’enrofloxacin e del ciprofloxacin su cellule dell’eritroleucemia murina.

A new mutagenesis test which uses the murine erythroleuke¬mia celI system was employed to evaluate the mutagen activity of enrofloxacin and its main metabolite, ciprofloxacin. After celI differentiation was induced by adding dimethyl sulfoxide (DMSO) to the culture medium, these xenobiotics were added to the culture medium in various doses and at different times. The mutagen activity of tested substances was calculated by determining the pertentage of cells capable of expressing the erythroid phenotype. The results show that enrofloxacin and ciprofloxacin did not decrease the capacity of MEL cells induced with DMSO to proliferate or differentiate into erythroid cells