Reduction of ferric hemoglobin from Trematomus bernacchii in a partial bis-histidyl state produces a deoxy coordination even when encapsulated into the crystal phase

Crystallographic and spectroscopic evidences on Antarctic fish hemoglobins (AFHbs) revealed that their ferric tetramers at physiological pH are in a mixed α(aquo-met)/β(bis-histidyl) coordination state and show a quaternary structure intermediate between the classical R and T states (H state). Ferric bis-histidyl adducts (hemichromes) have been also observed in some mammalian Hbs. In order to reveal if hemichrome in AFHbs can be converted into a hemochrome, at least in the crystal phase, chemical reduction on single crystals of ferric Hb from Trematomus bernacchii (HbTb) has been followed via Raman microscopy. The results of this analysis reveal that in HbTb, upon reduction, the bis-histidyl coordination is loosened in favor of a penta-coordinated ferrous deoxy state, with no evidence of ferrous bis-histidyl state. These data are in agreement with UV/Vis absorption spectra in solution. Furthermore, our data are also indirectly supported by the observation that upon reduction with dithionite, the ferric HbTb crystals crack and lose their diffraction power: in the crystalline state, the quaternary structure transition from the H to the T state is not compatible with the crystal packing. Altogether these data indicate that if bis-histidyl adducts have a functional significance in AFHbs, this function refers to a stable ferric state, or to a transient, though never detected, ferrous species.