The interest in DNA quadruplex structures has been fueled by the recognition that telomeres, the 3' single stranded guanine-rich overhangs found at the termini of chromosomes, are likely to form G-tetrads type structures important in cell senescence and cancer. In addn. to their presence in telomeres, where they may play a role in maintaining the stability and integrity of chromosomes, guanine-rich regions are found in other region of the genome, amongst these is intron 6 of hTERT a gene codifying for the enzyme telomerase. Interestingly, the formation of G-quadruplexes in this region is involved in the down-regulation of telomerase activity caused by an alteration of the hTERT splicing pattern. Therefore, we have analyzed several sequences of that intron by 1H-NMR and CD spectroscopy, and we have found that the sequence d(GGGGTGAAAGGGG) is able to fold in a single well-defined antiparallel quadruplex structure consisting of four G-tetrads, possessing a twofold symmetry, and contg. four Gs in a syn glycosidic conformation.

Structural insight into the hTERT intron 6 sequence d(GGGGTGAAAGGGG) from 1H-NMR study / A., Virno; Mayol, Luciano; A., Ramos; F., Fraternali; Pagano, Bruno; Randazzo, Antonio. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - STAMPA. - 26:8-9(2007), pp. 1133-1137. [10.1080/15257770701521854]

Structural insight into the hTERT intron 6 sequence d(GGGGTGAAAGGGG) from 1H-NMR study

MAYOL, LUCIANO;PAGANO, BRUNO;RANDAZZO, ANTONIO
2007

Abstract

The interest in DNA quadruplex structures has been fueled by the recognition that telomeres, the 3' single stranded guanine-rich overhangs found at the termini of chromosomes, are likely to form G-tetrads type structures important in cell senescence and cancer. In addn. to their presence in telomeres, where they may play a role in maintaining the stability and integrity of chromosomes, guanine-rich regions are found in other region of the genome, amongst these is intron 6 of hTERT a gene codifying for the enzyme telomerase. Interestingly, the formation of G-quadruplexes in this region is involved in the down-regulation of telomerase activity caused by an alteration of the hTERT splicing pattern. Therefore, we have analyzed several sequences of that intron by 1H-NMR and CD spectroscopy, and we have found that the sequence d(GGGGTGAAAGGGG) is able to fold in a single well-defined antiparallel quadruplex structure consisting of four G-tetrads, possessing a twofold symmetry, and contg. four Gs in a syn glycosidic conformation.
2007
Structural insight into the hTERT intron 6 sequence d(GGGGTGAAAGGGG) from 1H-NMR study / A., Virno; Mayol, Luciano; A., Ramos; F., Fraternali; Pagano, Bruno; Randazzo, Antonio. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - STAMPA. - 26:8-9(2007), pp. 1133-1137. [10.1080/15257770701521854]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/205529
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