The DNA sequence d(GGGGTTTTGGGG) consists of 1.5 units of the repeat in telomeres of Oxytricha nova. It has been shown by NMR and x-ray crystallog. anal. that it is capable to form a dimeric quadruplex structure and that a variety of cations, namely K+, Na+, and NH4+, are able to interact with this complex with different affinity, leading to complexes characterized by different local conformations. Thus, to improve the knowledge of this kind of mol., and in particular to provide further insight into the role of monovalent cations in the G-quadruplex folding and conformation, we have investigated by 1H-NMR the effect of the addn. of Rb+ and Cs+ to the quadruplex formed by the oligonucleotide d(GGGGTTTTGGGG).

Effect of Rubidium and Cesium Ions on The Dimeric Quaduplex Formed by the Oxytricha Nova Telomeric Repeat Oligonucleotide d(GGGGTTTTGGGG)

RANDAZZO, ANTONIO;
2007

Abstract

The DNA sequence d(GGGGTTTTGGGG) consists of 1.5 units of the repeat in telomeres of Oxytricha nova. It has been shown by NMR and x-ray crystallog. anal. that it is capable to form a dimeric quadruplex structure and that a variety of cations, namely K+, Na+, and NH4+, are able to interact with this complex with different affinity, leading to complexes characterized by different local conformations. Thus, to improve the knowledge of this kind of mol., and in particular to provide further insight into the role of monovalent cations in the G-quadruplex folding and conformation, we have investigated by 1H-NMR the effect of the addn. of Rb+ and Cs+ to the quadruplex formed by the oligonucleotide d(GGGGTTTTGGGG).
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11588/205528
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