Background and Objectives: Total parenteral nutrition (TPN) is a lifesaving therapy in children with intestinal failure, frequently complicated by liver dysfunction. Plant sterols (phytosterols) of lipid emulsions have been supposed to contribute to cholestasis in TPN-treated children. The present study aimed to evaluate the plasma and red blood cell membrane (RBCM) phytosterol levels in newborns after a short period of TPN. Patients and Methods: Phytosterols, cholesterol, and other sterol levels were quantified by gas chromatography-mass spectrometry in 15 healthy control infants, 22 patients after TPN, and 11 patients before TPN. Sterols of lipid emulsions were quantified. Results: Plasma and RBCM phytosterol levels were, respectively, on average 56mmol/L and 83mmol/g per protein in patients after TPN, 13mmol/L and 15mmol/g per protein in patients before TPN, and 9mmol/L and 13mmoL/g per protein in control infants (P<0.05 for differences). The days of TPN and the total amount of infused lipids correlated significantly with RBCM phytosterol (P<0.05); correlations for plasma were positive but not significant. No correlation was observed with plasma bilirubin, g-glutamyltransferase, or alanine transaminase. Conclusions: Plasma and RBCM phytosterols increase significantly in newborns after a short period of TPN. Higher phytosterol levels were observed in some patients that could have been due to their individual variability in phytosterol metabolism and/or clearance. A greater accumulation of phytosterols in membranes may induce TPN-related cholestasis.

Sterols profiling in red blood cell membranes and plasma of newborns receiving total parenteral nutrition / Pianese, P; Salvia, G; Campanozzi, A; Dapolito, O; DELLO RUSSO, Antonio; PETTOELLO MANTOVANI, M. AND CORSO G.. - In: JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION. - ISSN 0277-2116. - STAMPA. - 47:(2008), pp. 645-651.

Sterols profiling in red blood cell membranes and plasma of newborns receiving total parenteral nutrition.

DELLO RUSSO, ANTONIO;
2008

Abstract

Background and Objectives: Total parenteral nutrition (TPN) is a lifesaving therapy in children with intestinal failure, frequently complicated by liver dysfunction. Plant sterols (phytosterols) of lipid emulsions have been supposed to contribute to cholestasis in TPN-treated children. The present study aimed to evaluate the plasma and red blood cell membrane (RBCM) phytosterol levels in newborns after a short period of TPN. Patients and Methods: Phytosterols, cholesterol, and other sterol levels were quantified by gas chromatography-mass spectrometry in 15 healthy control infants, 22 patients after TPN, and 11 patients before TPN. Sterols of lipid emulsions were quantified. Results: Plasma and RBCM phytosterol levels were, respectively, on average 56mmol/L and 83mmol/g per protein in patients after TPN, 13mmol/L and 15mmol/g per protein in patients before TPN, and 9mmol/L and 13mmoL/g per protein in control infants (P<0.05 for differences). The days of TPN and the total amount of infused lipids correlated significantly with RBCM phytosterol (P<0.05); correlations for plasma were positive but not significant. No correlation was observed with plasma bilirubin, g-glutamyltransferase, or alanine transaminase. Conclusions: Plasma and RBCM phytosterols increase significantly in newborns after a short period of TPN. Higher phytosterol levels were observed in some patients that could have been due to their individual variability in phytosterol metabolism and/or clearance. A greater accumulation of phytosterols in membranes may induce TPN-related cholestasis.
2008
Sterols profiling in red blood cell membranes and plasma of newborns receiving total parenteral nutrition / Pianese, P; Salvia, G; Campanozzi, A; Dapolito, O; DELLO RUSSO, Antonio; PETTOELLO MANTOVANI, M. AND CORSO G.. - In: JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION. - ISSN 0277-2116. - STAMPA. - 47:(2008), pp. 645-651.
Sterols profiling in red blood cell membranes and plasma of newborns receiving total parenteral nutrition / Pianese, P; Salvia, G; Campanozzi, A; Dapolito, O; DELLO RUSSO, Antonio; PETTOELLO MANTOVANI, M. AND CORSO G.. - In: JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION. - ISSN 0277-2116. - STAMPA. - 47:(2008), pp. 645-651.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/205189
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