An easy and efficient solid-phase strategy to obtain 5¢- and 3¢-oligonucleotide conjugates in highly pure form has been developed. Ad hoc derivatized solid supports, to which the first nucleoside unit can be attached through a phosphate linkage, have been exploited both in a preand post-DNA assembly conjugation approach. A number of 5'- or 3'- oligonucleotide conjugates, incorporating a variety of labels covalently linked through a phosphodiester or a phosphoramidate bond, have been synthesized and characterized.
An efficient and versatile solid phase synthesis of 5'- and 3'-conjugated oligonucleotides / J., D'Onofrio; Montesarchio, Daniela; DE NAPOLI, Lorenzo; DI FABIO, Giovanni. - In: ORGANIC LETTERS. - ISSN 1523-7060. - ELETTRONICO. - 7:22(2005), pp. 4927-4930. [10.1021/ol051811g]
An efficient and versatile solid phase synthesis of 5'- and 3'-conjugated oligonucleotides
MONTESARCHIO, DANIELA;DE NAPOLI, LORENZO;DI FABIO, GIOVANNI
2005
Abstract
An easy and efficient solid-phase strategy to obtain 5¢- and 3¢-oligonucleotide conjugates in highly pure form has been developed. Ad hoc derivatized solid supports, to which the first nucleoside unit can be attached through a phosphate linkage, have been exploited both in a preand post-DNA assembly conjugation approach. A number of 5'- or 3'- oligonucleotide conjugates, incorporating a variety of labels covalently linked through a phosphodiester or a phosphoramidate bond, have been synthesized and characterized.File | Dimensione | Formato | |
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