MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.

A mammalian microRNA expression atlas based on small RNA library sequencing / Landgraf, P; Rusu, M; Sheridan, R; Sewer, A; Iovino, N; Aravin, A; Pfeffer, S; Rice, A; Kamphorst, Ao; Landthaler, M; Lin, C; Socci, Nd; Hermida, L; Fulci, V; Chiaretti, S; Foa, R; Schliwka, J; Fuchs, U; Novosel, A; Muller, Ru; Schermer, B; Bissels, U; Inman, J; Phan, Q; Chien, M; Weir, Db; Choksi, R; DE VITA, Gabriella; Frezzetti, D; Trompeter, Hi; Hornung, V; Teng, G; Hartmann, G; Palkovits, M; DI LAURO, Roberto; Wernet, P; Macino, G; Rogler, Ce; Nagle, Jw; Ju, J; Papavasiliou, Fn; Benzing, T; Lichter, P; Tam, W; Brownstein, Mj; Bosio, A; Borkhardt, A; Russo, Jj; Sander, C; Zavolan, M; Tuschl, T.. - In: CELL. - ISSN 0092-8674. - STAMPA. - 129:7(2007), pp. 1401-1414.

A mammalian microRNA expression atlas based on small RNA library sequencing.

DE VITA, GABRIELLA;DI LAURO, ROBERTO;
2007

Abstract

MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.
2007
A mammalian microRNA expression atlas based on small RNA library sequencing / Landgraf, P; Rusu, M; Sheridan, R; Sewer, A; Iovino, N; Aravin, A; Pfeffer, S; Rice, A; Kamphorst, Ao; Landthaler, M; Lin, C; Socci, Nd; Hermida, L; Fulci, V; Chiaretti, S; Foa, R; Schliwka, J; Fuchs, U; Novosel, A; Muller, Ru; Schermer, B; Bissels, U; Inman, J; Phan, Q; Chien, M; Weir, Db; Choksi, R; DE VITA, Gabriella; Frezzetti, D; Trompeter, Hi; Hornung, V; Teng, G; Hartmann, G; Palkovits, M; DI LAURO, Roberto; Wernet, P; Macino, G; Rogler, Ce; Nagle, Jw; Ju, J; Papavasiliou, Fn; Benzing, T; Lichter, P; Tam, W; Brownstein, Mj; Bosio, A; Borkhardt, A; Russo, Jj; Sander, C; Zavolan, M; Tuschl, T.. - In: CELL. - ISSN 0092-8674. - STAMPA. - 129:7(2007), pp. 1401-1414.
File in questo prodotto:
File Dimensione Formato  
Landgraf et al.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: Accesso privato/ristretto
Dimensione 1.35 MB
Formato Adobe PDF
1.35 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/204225
Citazioni
  • ???jsp.display-item.citation.pmc??? 1852
  • Scopus 3134
  • ???jsp.display-item.citation.isi??? 2978
social impact