An essential but insufficient step for apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) in epithelial cells is their association with detergent-resistant microdomains (DRMs) or rafts. In this paper, we show that in MDCK cells both apical and basolateral GPI-APs associate with DRMs during their biosynthesis. However, only apical and not basolateral GPI-APs are able to oligomerize into high molecular weight complexes. Protein oligomerization begins in the medial Golgi, concomitantly with DRM association, and is dependent on protein-protein interactions. Impairment of oligomerization leads to protein missorting. We propose that oligomerization stabilizes GPI-APs into rafts and that this additional step is required for apical sorting of GPI-APs. Two alternative apical sorting models are presented.

Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins / Paladino, Simona; Sarnataro, Daniela; Pillich, R; Tivodar, S; Nitsch, Lucio; Zurzolo, Chiara. - In: THE JOURNAL OF CELL BIOLOGY. - ISSN 0021-9525. - STAMPA. - 167:4(2004), pp. 699-709. [10.1083/jcb.200407094]

Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins.

PALADINO, SIMONA;SARNATARO, DANIELA;NITSCH, LUCIO;ZURZOLO, CHIARA
2004

Abstract

An essential but insufficient step for apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) in epithelial cells is their association with detergent-resistant microdomains (DRMs) or rafts. In this paper, we show that in MDCK cells both apical and basolateral GPI-APs associate with DRMs during their biosynthesis. However, only apical and not basolateral GPI-APs are able to oligomerize into high molecular weight complexes. Protein oligomerization begins in the medial Golgi, concomitantly with DRM association, and is dependent on protein-protein interactions. Impairment of oligomerization leads to protein missorting. We propose that oligomerization stabilizes GPI-APs into rafts and that this additional step is required for apical sorting of GPI-APs. Two alternative apical sorting models are presented.
2004
Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins / Paladino, Simona; Sarnataro, Daniela; Pillich, R; Tivodar, S; Nitsch, Lucio; Zurzolo, Chiara. - In: THE JOURNAL OF CELL BIOLOGY. - ISSN 0021-9525. - STAMPA. - 167:4(2004), pp. 699-709. [10.1083/jcb.200407094]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/203334
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