In this study, we report that the human p14(ARF) associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14(ARF) over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14(ARF) are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14(ARF) protein is involved in binding to c-Myc and N-Myc proteins.
p14ARF interacts with N-Myc and inhibits its transcriptional activity / Amente, Stefano; Gargano, Barbara; Diolaiti, D; DELLA VALLE, G; Lania, Luigi; Majello, Barbara. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 581:(2007), pp. 821-825.
p14ARF interacts with N-Myc and inhibits its transcriptional activity.
AMENTE, STEFANO;GARGANO, BARBARA;LANIA, LUIGI;MAJELLO, BARBARA
2007
Abstract
In this study, we report that the human p14(ARF) associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14(ARF) over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14(ARF) are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14(ARF) protein is involved in binding to c-Myc and N-Myc proteins.File | Dimensione | Formato | |
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