CLONING AND MOLECULAR CHARACTERIZATION OF THE FIRST INNEXIN OF THE PHILUM ANNELIDA-ESPRESSION OF THE GENE DURING DEVELOPMENT

A novel member of the innexin family (cvinx) has been isolated from the annelid polychaete worm ChaetopterusvariopedatususingaPCRapproachongenomic DNA and sequence analysis on genomic DNA clones. The gene is present in aHindIII-HindIII segment of 2250 bp containing an uninterrupted open reading frame of 1196 bp encoding a protein of 399 amino acids. The predicted protein shows the typical structural features of innexins and consensus sites for phosphorylation. Analyses on genomic DNA demonstrate that cv-inx is a single copy gene with no introns in the coding region, exactly corresponding to the cDNA sequence. The gene expression is regulated during development as shown by Northern blots analyses of the RNA and by immunoreaction with antibodies against the protein at several embryonic stages. The finding of an innexin in the phylum Annelida, outside of the Ecdysozoa clade, and its peculiar gene structure suggest the necessity to reconsider the current hypothesis on the origin and evolution of gap junctional proteins.