Human spermatozoa are more dependent on glutathione peroxidase/glutathione reductase (GPX/GR) system, via reduced glutathione (GSH), to inactivate reactive oxygen metabolites (ROMs) such as hydrogen peroxide and organic hydroperoxides. To demonstrate whether there is a substantial difference in GPX activity between normal and pathological seminal plasma, we decided to evaluate the activity of this enzyme in healthy subjects and infertile males with normal hormonal patterns. Our results demonstrate that in healthy subjects the seminal plasma contains a GPX activity that is about 10 times greater than the GPX activity detected in the seminal plasma of infertile males. By using specific antibodies against plasmatic GPX (GPX3), we also demonstrate that enzymatic activity, detected in the seminal plasma of both healthy and infertile males is GPX3. Therefore, the evaluation of GPX activity in human seminal plasma could be a new useful marker of gonadal function in men

GLUTATHIONE PEROXIDASE (GPX) ACTIVITY IN SEMINAL PLASMA OF HEALTHY AND INFERTILE MALES / A., Giannattasio; M., DE ROSA; R., Smeraglia; Zarrilli, Stefano; A., Cimmino; B., DI ROSARIO; R., Ruggiero; A., Colao; G., Lombardi. - In: JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. - ISSN 0391-4097. - ELETTRONICO. - 25:(2002), pp. 983-986.

GLUTATHIONE PEROXIDASE (GPX) ACTIVITY IN SEMINAL PLASMA OF HEALTHY AND INFERTILE MALES

ZARRILLI, STEFANO;
2002

Abstract

Human spermatozoa are more dependent on glutathione peroxidase/glutathione reductase (GPX/GR) system, via reduced glutathione (GSH), to inactivate reactive oxygen metabolites (ROMs) such as hydrogen peroxide and organic hydroperoxides. To demonstrate whether there is a substantial difference in GPX activity between normal and pathological seminal plasma, we decided to evaluate the activity of this enzyme in healthy subjects and infertile males with normal hormonal patterns. Our results demonstrate that in healthy subjects the seminal plasma contains a GPX activity that is about 10 times greater than the GPX activity detected in the seminal plasma of infertile males. By using specific antibodies against plasmatic GPX (GPX3), we also demonstrate that enzymatic activity, detected in the seminal plasma of both healthy and infertile males is GPX3. Therefore, the evaluation of GPX activity in human seminal plasma could be a new useful marker of gonadal function in men
2002
GLUTATHIONE PEROXIDASE (GPX) ACTIVITY IN SEMINAL PLASMA OF HEALTHY AND INFERTILE MALES / A., Giannattasio; M., DE ROSA; R., Smeraglia; Zarrilli, Stefano; A., Cimmino; B., DI ROSARIO; R., Ruggiero; A., Colao; G., Lombardi. - In: JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. - ISSN 0391-4097. - ELETTRONICO. - 25:(2002), pp. 983-986.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/169475
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