In the present study, by comparing the responses in wild-type (WT) mice and mice lacking [knockout (KO)] the 5-lipoxygenase (5-LO), we investigated the role played by 5-LO in the development of acute inflammation. When compared with carragenan-treated 5-LOWT mice, 5-LOKO mice, which had received carrageenan, exhibited a reduced degree of pleural exudation, polymorphonuclear cell migration. Lung myeloperoxidase activity, an index of neutrophil infiltration, was significantly reduced in 5-LOKO mice in comparison with 5-LOWT. Lung-tissue sections from carrageenan-treated 5-LOWT mice showed positive staining for intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), P-selectin, and E-selectin, which were mainly localized around vessels. The intensity and degree of ICAM-1, VCAM-1, P-selectin, and E-selectin were markedly reduced in tissue section from carrageenan-5-LOKO mice, which also improved the histological status of the inflamed lungs. Taken together, our results clearly demonstrate that 5-LO modulates neutrophil infiltration in the acute lung inflammation.

5-LIPOXYGENASE KNOCKOUT MICE EXHIBIT A RESISTANCE TO PLEURISY AND LUNG INJURY CAUSED BY CARRAGEENAN / Cuzzocrea, S.; Rossi, Antonietta; Serraino, I.; Mazzon, E.; DI PAOLA, R.; Dugo, L.; Genovese, T.; Calabro, B.; Caputi, A. P.; Sautebin, Lidia. - In: JOURNAL OF LEUKOCYTE BIOLOGY. - ISSN 0741-5400. - 73:6(2003), pp. 739-746. [10.1189/jlb.1002477]

5-LIPOXYGENASE KNOCKOUT MICE EXHIBIT A RESISTANCE TO PLEURISY AND LUNG INJURY CAUSED BY CARRAGEENAN.

ROSSI, ANTONIETTA;SAUTEBIN, LIDIA
2003

Abstract

In the present study, by comparing the responses in wild-type (WT) mice and mice lacking [knockout (KO)] the 5-lipoxygenase (5-LO), we investigated the role played by 5-LO in the development of acute inflammation. When compared with carragenan-treated 5-LOWT mice, 5-LOKO mice, which had received carrageenan, exhibited a reduced degree of pleural exudation, polymorphonuclear cell migration. Lung myeloperoxidase activity, an index of neutrophil infiltration, was significantly reduced in 5-LOKO mice in comparison with 5-LOWT. Lung-tissue sections from carrageenan-treated 5-LOWT mice showed positive staining for intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), P-selectin, and E-selectin, which were mainly localized around vessels. The intensity and degree of ICAM-1, VCAM-1, P-selectin, and E-selectin were markedly reduced in tissue section from carrageenan-5-LOKO mice, which also improved the histological status of the inflamed lungs. Taken together, our results clearly demonstrate that 5-LO modulates neutrophil infiltration in the acute lung inflammation.
2003
5-LIPOXYGENASE KNOCKOUT MICE EXHIBIT A RESISTANCE TO PLEURISY AND LUNG INJURY CAUSED BY CARRAGEENAN / Cuzzocrea, S.; Rossi, Antonietta; Serraino, I.; Mazzon, E.; DI PAOLA, R.; Dugo, L.; Genovese, T.; Calabro, B.; Caputi, A. P.; Sautebin, Lidia. - In: JOURNAL OF LEUKOCYTE BIOLOGY. - ISSN 0741-5400. - 73:6(2003), pp. 739-746. [10.1189/jlb.1002477]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/165484
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