Thyroid cells transformed by the Kirsten-ras oncogene become tumorigenic in syngeneic animals. Their growth is no longer dependent on TSH but becomes dependent on serum. Combining morphological and biochemical evidence, we show that serum withdrawal induces apoptotic cell death in Kirsten and Harvey-ras transformed thyroid cell. On the other hand, neither serum nor TSH withdrawal induce apoptosis in differentiated FRTL-5 cells. The induction of apoptosis by serum withdrawal is rapid and not triggered at a specific phase of the cell cycle. We suggest that induction of apoptosis following growth factor deprivation is an additional important characteristic, besides TSH-independence for growth and dedifferentiation, of the thyroid transformed phenotype.

Serum withdrawal induces apoptotic cell death in Ki-ras transformed but not in normal differentiated thyroid cells / DI JESO, B; Ulianich, L; Racioppi, Luigi; D'Armiento, FRANCESCO PAOLO; Feliciello, Antonio; Pacifico, F; Consiglio, E; Formisano, Silvestro. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 214(3):(1995), pp. 819-824. [10.1006/bbrc.1995.2360]

Serum withdrawal induces apoptotic cell death in Ki-ras transformed but not in normal differentiated thyroid cells.

RACIOPPI, LUIGI;D'ARMIENTO, FRANCESCO PAOLO;FELICIELLO, ANTONIO;FORMISANO, SILVESTRO
1995

Abstract

Thyroid cells transformed by the Kirsten-ras oncogene become tumorigenic in syngeneic animals. Their growth is no longer dependent on TSH but becomes dependent on serum. Combining morphological and biochemical evidence, we show that serum withdrawal induces apoptotic cell death in Kirsten and Harvey-ras transformed thyroid cell. On the other hand, neither serum nor TSH withdrawal induce apoptosis in differentiated FRTL-5 cells. The induction of apoptosis by serum withdrawal is rapid and not triggered at a specific phase of the cell cycle. We suggest that induction of apoptosis following growth factor deprivation is an additional important characteristic, besides TSH-independence for growth and dedifferentiation, of the thyroid transformed phenotype.
1995
Serum withdrawal induces apoptotic cell death in Ki-ras transformed but not in normal differentiated thyroid cells / DI JESO, B; Ulianich, L; Racioppi, Luigi; D'Armiento, FRANCESCO PAOLO; Feliciello, Antonio; Pacifico, F; Consiglio, E; Formisano, Silvestro. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 214(3):(1995), pp. 819-824. [10.1006/bbrc.1995.2360]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/159812
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact