Telomeric guanine-rich sequence can adopt quadruplex structures that are important for their biological role in chromosomal stabilisation. G quartets are characterised by the cyclic hydrogen bonding of four guanine bases in a coplanar arrangement and their stability is ion-dependent. In this work we compare the stability of [d(TGGGT)](4) and [d(T*GGGT)](4) quadruplexes. The last one contains a modified thymine, where the hydroxyl group substitutes one hydrogen atom of the methyl group of the thymine in the [d(TGGGT)](4) sequence. We used a combination of spectroscopic, calorimetric and computational techniques to characterise the G-quadruplex formation. NMR and CD spectra of [d(T*GGGT)](4) were characteristic of parallel-stranded, tetramolecular quadruplex. CD and DSC melting experiments reveal that [d(T*GGGT)](4) is less stable that unmodified quadruplex. Molecular models suggest possible explanation for the observed behaviour.

Effect of a modified thymine on the structure and stability of [d(TGGGT)]4 quadruplex / Petraccone, Luigi; Erra, E; Nasti, L; Galeone, Aldo; Randazzo, Antonio; Mayol, Luciano; Barone, Guido; Giancola, Concetta. - In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. - ISSN 0141-8130. - STAMPA. - 31:4-5(2003), pp. 131-137. [10.1016/S0141-8130(02)00073-9]

Effect of a modified thymine on the structure and stability of [d(TGGGT)]4 quadruplex

PETRACCONE, LUIGI;GALEONE, ALDO;RANDAZZO, ANTONIO;MAYOL, LUCIANO;BARONE, GUIDO;GIANCOLA, CONCETTA
2003

Abstract

Telomeric guanine-rich sequence can adopt quadruplex structures that are important for their biological role in chromosomal stabilisation. G quartets are characterised by the cyclic hydrogen bonding of four guanine bases in a coplanar arrangement and their stability is ion-dependent. In this work we compare the stability of [d(TGGGT)](4) and [d(T*GGGT)](4) quadruplexes. The last one contains a modified thymine, where the hydroxyl group substitutes one hydrogen atom of the methyl group of the thymine in the [d(TGGGT)](4) sequence. We used a combination of spectroscopic, calorimetric and computational techniques to characterise the G-quadruplex formation. NMR and CD spectra of [d(T*GGGT)](4) were characteristic of parallel-stranded, tetramolecular quadruplex. CD and DSC melting experiments reveal that [d(T*GGGT)](4) is less stable that unmodified quadruplex. Molecular models suggest possible explanation for the observed behaviour.
2003
Effect of a modified thymine on the structure and stability of [d(TGGGT)]4 quadruplex / Petraccone, Luigi; Erra, E; Nasti, L; Galeone, Aldo; Randazzo, Antonio; Mayol, Luciano; Barone, Guido; Giancola, Concetta. - In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. - ISSN 0141-8130. - STAMPA. - 31:4-5(2003), pp. 131-137. [10.1016/S0141-8130(02)00073-9]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/158589
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