H-1-NMR, CD, and UV spectroscopy have been used to investigate the structure of PNA/DNA chimeras forming quadruplex structures. In particular, we synthesized (5')TGGG(3')-t (1) and (5')TGG(3')-gt (2), where lower and upper case letters indicate PNA and DNA residues, respectively. CD spectrum and all NMR data of (1) are typical of quadruplexes involving four parallel strands. UV melting profile of (1) indicates that its thermal stability is quite similar to that observed for the reference structure [d(TGGGT)](4). H-1-NMR spectrum for (5')TGG(3')-gt (2) shows that this oligonucleotide is not able to fold into a single, well-defined species.

PNA-DNA chimeras forming quadruplex structures / Esposito, Veronica; Galeone, Aldo; Mayol, Luciano; A., Messere; Piccialli, Gennaro; Randazzo, Antonio. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - 22:(2003), pp. 1681-1684. [10.1081/NCN-120023112]

PNA-DNA chimeras forming quadruplex structures

ESPOSITO, VERONICA;GALEONE, ALDO;MAYOL, LUCIANO;PICCIALLI, GENNARO;RANDAZZO, ANTONIO
2003

Abstract

H-1-NMR, CD, and UV spectroscopy have been used to investigate the structure of PNA/DNA chimeras forming quadruplex structures. In particular, we synthesized (5')TGGG(3')-t (1) and (5')TGG(3')-gt (2), where lower and upper case letters indicate PNA and DNA residues, respectively. CD spectrum and all NMR data of (1) are typical of quadruplexes involving four parallel strands. UV melting profile of (1) indicates that its thermal stability is quite similar to that observed for the reference structure [d(TGGGT)](4). H-1-NMR spectrum for (5')TGG(3')-gt (2) shows that this oligonucleotide is not able to fold into a single, well-defined species.
2003
PNA-DNA chimeras forming quadruplex structures / Esposito, Veronica; Galeone, Aldo; Mayol, Luciano; A., Messere; Piccialli, Gennaro; Randazzo, Antonio. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - 22:(2003), pp. 1681-1684. [10.1081/NCN-120023112]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/155370
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 8
social impact