In order to provide cytological evidence of amplification, Podarcis sicula oocytes were studied by cytophotometry, thymidine incorporation and in situ DNA-DNA hybridization. Our results show that DNA replication is completed during the preleptotene stage, the leptotene oocytes having the typical 4C nuclear DNA content. Between the zygotene and the mid-pachytene stages further DNA synthesis occurs with consequent increase of the ribosomal nuclear DNA content. These results and the variations in nucleolar organization observed during differentiation give clear evidence of the existence of ribosomal gene amplification in Podarcis sicula oocytes.

Ribosomal gene amplification in oocytes of the lizard Podarcis sicula / Motta, CHIARA MARIA; Andreuccetti, Piero; Filosa, Silvana. - In: MOLECULAR REPRODUCTION AND DEVELOPMENT. - ISSN 1040-452X. - STAMPA. - 29:(1991), pp. 95-102. [10.1002/mrd.1080290202]

Ribosomal gene amplification in oocytes of the lizard Podarcis sicula.

MOTTA, CHIARA MARIA;ANDREUCCETTI, PIERO;FILOSA, SILVANA
1991

Abstract

In order to provide cytological evidence of amplification, Podarcis sicula oocytes were studied by cytophotometry, thymidine incorporation and in situ DNA-DNA hybridization. Our results show that DNA replication is completed during the preleptotene stage, the leptotene oocytes having the typical 4C nuclear DNA content. Between the zygotene and the mid-pachytene stages further DNA synthesis occurs with consequent increase of the ribosomal nuclear DNA content. These results and the variations in nucleolar organization observed during differentiation give clear evidence of the existence of ribosomal gene amplification in Podarcis sicula oocytes.
1991
Ribosomal gene amplification in oocytes of the lizard Podarcis sicula / Motta, CHIARA MARIA; Andreuccetti, Piero; Filosa, Silvana. - In: MOLECULAR REPRODUCTION AND DEVELOPMENT. - ISSN 1040-452X. - STAMPA. - 29:(1991), pp. 95-102. [10.1002/mrd.1080290202]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/152655
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