To determine whether oogonial proliferation and oocyte recruitment are under control of hypophyseal and/or ovarian factors, we carried out a series of investigations using Podarcis sicula, a lizard inhabiting the temperate lowlands of Europe in which oocyte recruitment occurs throughout the year, as animal model. Germinal beds containing oogonia and oocytes in prefollicular stages were cocultured with different ovarian compartments in presence/absence of FSH, and the effects of different treatments were evaluated by counting the number of prelepto-leptotene oocytes. Results revealed that oocyte recruitment from the pool of oogonia is under the control of a factor released by follicle cells while FSH has an indirect effect on modulating oogonial proliferation. SDS-PAGE analyses carried out on media conditioned by follicles suggest that the factor involved in the control of oocyte recruitment may be a small protein (about 21 kDa) and that its release is dependent on the period of the ovarian cycle but apparently not on the circulating levels of FSH.

Control of oocyte recruitment: regulative role of follicle cells through the release of a diffusible factor / Sica, Stefania; Fierro, D; Iodice, C; Muoio, R; Filosa, Silvana; Motta, CHIARA MARIA. - In: MOLECULAR REPRODUCTION AND DEVELOPMENT. - ISSN 1040-452X. - STAMPA. - 58:(2001), pp. 444-450.

Control of oocyte recruitment: regulative role of follicle cells through the release of a diffusible factor.

SICA, STEFANIA;FILOSA, SILVANA;MOTTA, CHIARA MARIA
2001

Abstract

To determine whether oogonial proliferation and oocyte recruitment are under control of hypophyseal and/or ovarian factors, we carried out a series of investigations using Podarcis sicula, a lizard inhabiting the temperate lowlands of Europe in which oocyte recruitment occurs throughout the year, as animal model. Germinal beds containing oogonia and oocytes in prefollicular stages were cocultured with different ovarian compartments in presence/absence of FSH, and the effects of different treatments were evaluated by counting the number of prelepto-leptotene oocytes. Results revealed that oocyte recruitment from the pool of oogonia is under the control of a factor released by follicle cells while FSH has an indirect effect on modulating oogonial proliferation. SDS-PAGE analyses carried out on media conditioned by follicles suggest that the factor involved in the control of oocyte recruitment may be a small protein (about 21 kDa) and that its release is dependent on the period of the ovarian cycle but apparently not on the circulating levels of FSH.
2001
Control of oocyte recruitment: regulative role of follicle cells through the release of a diffusible factor / Sica, Stefania; Fierro, D; Iodice, C; Muoio, R; Filosa, Silvana; Motta, CHIARA MARIA. - In: MOLECULAR REPRODUCTION AND DEVELOPMENT. - ISSN 1040-452X. - STAMPA. - 58:(2001), pp. 444-450.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/152651
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