We report a novel method for preparing samples for laser capture microdissection. The procedure described here permits extraction of intact RNA while preserving morphology, thus being suitable both for identification of specific cells and for analysis of their gene expression. The method is applicable to both mouse embryos and human tumors and may improve the preparation of cDNA libraries from specific cell types without interfering with histological diagnosis. (c)2001 Elsevier Science.

A preservation method that allows recovery of intact RNA from tissues disected by laser capture microdissection / Parlato, Rosanna; Rosica, Annamaria; Cuccurullo, Vincenzo; Mansi, Luigi; Macchia, PAOLO EMIDIO; Owens, J. D.; Mushinski, J. F.; DE FELICE, Mario; Bonner, R. F.; DI LAURO, Roberto. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - STAMPA. - 300:(2002), pp. 139-145.

A preservation method that allows recovery of intact RNA from tissues disected by laser capture microdissection

PARLATO, ROSANNA;ROSICA, ANNAMARIA;CUCCURULLO, VINCENZO;MANSI, LUIGI;MACCHIA, PAOLO EMIDIO;DE FELICE, MARIO;DI LAURO, ROBERTO
2002

Abstract

We report a novel method for preparing samples for laser capture microdissection. The procedure described here permits extraction of intact RNA while preserving morphology, thus being suitable both for identification of specific cells and for analysis of their gene expression. The method is applicable to both mouse embryos and human tumors and may improve the preparation of cDNA libraries from specific cell types without interfering with histological diagnosis. (c)2001 Elsevier Science.
2002
A preservation method that allows recovery of intact RNA from tissues disected by laser capture microdissection / Parlato, Rosanna; Rosica, Annamaria; Cuccurullo, Vincenzo; Mansi, Luigi; Macchia, PAOLO EMIDIO; Owens, J. D.; Mushinski, J. F.; DE FELICE, Mario; Bonner, R. F.; DI LAURO, Roberto. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - STAMPA. - 300:(2002), pp. 139-145.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/149231
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