Homonuclear NMR techniques have been used to investigate the interactions of the minor groove binding agents distamycin A (Dist-A) and the related drug netropsin (Net) with three quadruplexes characterized by different groove widths: [d(TGGGGT)](4) (Q1), [d(GGGGTTTTGGGG)](2) (Q2), and d(GGGGTTGGGGTGTGGGGTTGGGG) (Q3). Netropsin has been found to be in a fast chemical exchange with all three kinds of quadruplexes, whereas Dist-A interacts tightly with Q1 and, at a less extent, with Q2. In order to determine the degree of selectivity of Dist-A for two- rather than four-stranded DNA, we titrated with Dist-A an equimolar solution of Q1 and the duplex d(CGCAAATTTGCG)(2) (D). This comparative H-1-NMR study allowed us to conclude that Dist-A and, consequently, Net possess higher affinity for duplex DNA.
Interaction of Distamycin A and Netropsin with Quadruplex and Duplex Structures: a Comparative 1H-NMR study / Randazzo, Antonio; Galeone, Aldo; Esposito, Veronica; Varra, Michela; Mayol, Luciano. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - STAMPA. - 21:8-9(2002), pp. 535-545. [10.1081/NCN-120015067]
Interaction of Distamycin A and Netropsin with Quadruplex and Duplex Structures: a Comparative 1H-NMR study
RANDAZZO, ANTONIO;GALEONE, ALDO;ESPOSITO, VERONICA;VARRA, MICHELA;MAYOL, LUCIANO
2002
Abstract
Homonuclear NMR techniques have been used to investigate the interactions of the minor groove binding agents distamycin A (Dist-A) and the related drug netropsin (Net) with three quadruplexes characterized by different groove widths: [d(TGGGGT)](4) (Q1), [d(GGGGTTTTGGGG)](2) (Q2), and d(GGGGTTGGGGTGTGGGGTTGGGG) (Q3). Netropsin has been found to be in a fast chemical exchange with all three kinds of quadruplexes, whereas Dist-A interacts tightly with Q1 and, at a less extent, with Q2. In order to determine the degree of selectivity of Dist-A for two- rather than four-stranded DNA, we titrated with Dist-A an equimolar solution of Q1 and the duplex d(CGCAAATTTGCG)(2) (D). This comparative H-1-NMR study allowed us to conclude that Dist-A and, consequently, Net possess higher affinity for duplex DNA.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
