An improved protocol for the synthesis of oligonucleotides containing a 3′−3′ phosphodiester linkage, for use in an alternate strand triple-helix formation approach, is reported. Three 16-mers and one 24-mer have been efficiently synthesized and the corresponding triplexes have been analyzed by thermal denaturation experiments and CD spectroscopy.
A facile solid-phase syntesis of oligonucleotides containing a 3’-3’ phosphodiester bond for alternate strand triple-helix formation / DE NAPOLI, Lorenzo; DI FABIO, Giovanni; Messere, A.; Montesarchio, Daniela; Piccialli, Gennaro; Varra, Michela. - In: EUROPEAN JOURNAL OF ORGANIC CHEMISTRY. - ISSN 1434-193X. - ELETTRONICO. - 1998:(1998), pp. 2119-2125. [10.1002/(SICI)1099-0690(199810)1998:10<2119::AID-EJOC2119>3.0.CO;2-K]
A facile solid-phase syntesis of oligonucleotides containing a 3’-3’ phosphodiester bond for alternate strand triple-helix formation
DE NAPOLI, LORENZO;DI FABIO, GIOVANNI;MONTESARCHIO, DANIELA;PICCIALLI, GENNARO;VARRA, MICHELA
1998
Abstract
An improved protocol for the synthesis of oligonucleotides containing a 3′−3′ phosphodiester linkage, for use in an alternate strand triple-helix formation approach, is reported. Three 16-mers and one 24-mer have been efficiently synthesized and the corresponding triplexes have been analyzed by thermal denaturation experiments and CD spectroscopy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
