Two novel alleles at the goat CSN1S2 locus have been identified: CSN1S2(F) and CSN1S2(D). Sequence analyses revealed that the CSN1S2(F) allele is characterized by a G --> A transition at the 13th nucleotide in exon 3 changing the seventh amino acid of the mature protein from Val to Ile. The CSN1S2(D) allele, apparently associated with a decreased synthesis of alpha s2-casein, is characterized by a 106-bp deletion, involving the last 11 bp of the exon 11 and the first 95 bp of the following intron. Methods (PCR-RFLP and PCR) for identification of carriers of these alleles have been developed.
Characterization of two new alleles at the goat CSN1S2 locus / Ramunno, Luigi; Cosenza, Gianfranco; Pappalardo, Margherita; Longobardi, E; Gallo, Daniela; Pastore, N; DI GREGORIO, P; Rando, A.. - In: ANIMAL GENETICS. - ISSN 0268-9146. - 32 (5):(2001), pp. 264-268. [10.1046/j.1365-2052.2001.00786.x]
Characterization of two new alleles at the goat CSN1S2 locus.
RAMUNNO, LUIGI;COSENZA, GIANFRANCO;PAPPALARDO, MARGHERITA;GALLO, DANIELA;
2001
Abstract
Two novel alleles at the goat CSN1S2 locus have been identified: CSN1S2(F) and CSN1S2(D). Sequence analyses revealed that the CSN1S2(F) allele is characterized by a G --> A transition at the 13th nucleotide in exon 3 changing the seventh amino acid of the mature protein from Val to Ile. The CSN1S2(D) allele, apparently associated with a decreased synthesis of alpha s2-casein, is characterized by a 106-bp deletion, involving the last 11 bp of the exon 11 and the first 95 bp of the following intron. Methods (PCR-RFLP and PCR) for identification of carriers of these alleles have been developed.| File | Dimensione | Formato | |
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