A procedure is described for the selection of mouse Lyt2- mutant cells. The procedure is based upon repeated cycles of selection with rat monoclonal antibodies to the Lyt2 antigen and magnetic particles coated with goat anti-mouse IgG. Stable Lyt2- mutant clones were derived from cells previously mutagenized with X rays and, at a lower frequency, also from non-mutagenized cells. The procedure can be used to rescue Lyt2- cells when the ratio of Lyt2+:Lyt2- cells is 10(4):1.
Isolation of mouse Lyt2- mutant cells using magnetic particles / Iannelli, D.; Capparelli, Rosanna; Scala, Felice. - In: JOURNAL OF IMMUNOLOGICAL METHODS. - ISSN 0022-1759. - ELETTRONICO. - 154:(1992), pp. 211-216.
Isolation of mouse Lyt2- mutant cells using magnetic particles
CAPPARELLI, ROSANNA;SCALA, FELICE
1992
Abstract
A procedure is described for the selection of mouse Lyt2- mutant cells. The procedure is based upon repeated cycles of selection with rat monoclonal antibodies to the Lyt2 antigen and magnetic particles coated with goat anti-mouse IgG. Stable Lyt2- mutant clones were derived from cells previously mutagenized with X rays and, at a lower frequency, also from non-mutagenized cells. The procedure can be used to rescue Lyt2- cells when the ratio of Lyt2+:Lyt2- cells is 10(4):1.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
