Rats injected in the hind paw with a mixture of Mycobacterium butirricum emulsified in mineral oil (FA) developed a severe polyarthritis that shared some immunological features with human rheumatoid arthritis. After this local administration, rats developed a secondary lesion (edema) in the contralateral paw, which is a hallmark of immune system activation. In vivo intravenous treatment with a monoclonal anti-very late antigen (VLA)-1 antibody (HA31/8) significantly reduced the edema formation in the contralateral paw. T cells isolated from contralateral paw draining lymph nodes of FA rats treated with HA31/8 showed a reduced cell proliferation in vitro, after stimulation with concanavalin A. Furthermore FACS analysis showed that the reduction in proliferation was concomitant to a reduction in the number of T cells positive to surface IL-2 receptor expression. Our data indicate that after in vivo treatment with a monoclonal anti-very late antigen-1 antibody, there is a beneficial effect on the development of the secondary lesion, which correlates to the reduced ability of T cells to proliferate in vitro as well as to a reduced surface expression of IL-2 receptor. The association of this antibody to other drugs interfering at other levels in rheumatoid arthritis may open a new therapeutic window.

Anti-Very Late Antigen-1 monoclonal antibody modulates the development of secondary lesion and T-cell response in experimental arthritis / Ianaro, Angela; Cicala, Carla; Calignano, Antonio; Koteliansky, V; Gotwals, P; Bucci, Mariarosaria; Gerli, R; Santucci, L; Fiorucci, S; Cirino, Giuseppe. - In: LABORATORY INVESTIGATION. - ISSN 0023-6837. - STAMPA. - 80:1(2000), pp. 73-80. [10.1038/labinvest.3780010]

Anti-Very Late Antigen-1 monoclonal antibody modulates the development of secondary lesion and T-cell response in experimental arthritis.

IANARO, ANGELA;CICALA, CARLA;CALIGNANO, ANTONIO;BUCCI, MARIAROSARIA;CIRINO, GIUSEPPE
2000

Abstract

Rats injected in the hind paw with a mixture of Mycobacterium butirricum emulsified in mineral oil (FA) developed a severe polyarthritis that shared some immunological features with human rheumatoid arthritis. After this local administration, rats developed a secondary lesion (edema) in the contralateral paw, which is a hallmark of immune system activation. In vivo intravenous treatment with a monoclonal anti-very late antigen (VLA)-1 antibody (HA31/8) significantly reduced the edema formation in the contralateral paw. T cells isolated from contralateral paw draining lymph nodes of FA rats treated with HA31/8 showed a reduced cell proliferation in vitro, after stimulation with concanavalin A. Furthermore FACS analysis showed that the reduction in proliferation was concomitant to a reduction in the number of T cells positive to surface IL-2 receptor expression. Our data indicate that after in vivo treatment with a monoclonal anti-very late antigen-1 antibody, there is a beneficial effect on the development of the secondary lesion, which correlates to the reduced ability of T cells to proliferate in vitro as well as to a reduced surface expression of IL-2 receptor. The association of this antibody to other drugs interfering at other levels in rheumatoid arthritis may open a new therapeutic window.
2000
Anti-Very Late Antigen-1 monoclonal antibody modulates the development of secondary lesion and T-cell response in experimental arthritis / Ianaro, Angela; Cicala, Carla; Calignano, Antonio; Koteliansky, V; Gotwals, P; Bucci, Mariarosaria; Gerli, R; Santucci, L; Fiorucci, S; Cirino, Giuseppe. - In: LABORATORY INVESTIGATION. - ISSN 0023-6837. - STAMPA. - 80:1(2000), pp. 73-80. [10.1038/labinvest.3780010]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/132865
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