Glu85 in the Escherichia coli thioredoxin, which is localized in the loop between beta4 and beta5, was substituted with the Arg present in the corresponding position in Bacillus acidocaldarius thioredoxin. This suggested that it could play an important role in the structure and thermostability of this protein owing to its involvement in numerous interactions. The effects of the mutation on the biophysical properties were analysed by circular dichroism, spectrofluorimetry and limited proteolysis, supported by molecular dynamics data. As modelling predicted, an increase in stability for E85R due to additional H-bonds between the beta5 and alpha4 regions was observed.

A single point mutation (Glu85Arg) increases the stability of the thioredoxin from Escherichia coli / Pedone, E.; Saviano, M.; Rossi, Mose'; Bartolucci, Simonetta. - In: PROTEIN ENGINEERING. - ISSN 0269-2139. - STAMPA. - 14:4(2001), pp. 255-260. [10.1093/protein/14.4.255]

A single point mutation (Glu85Arg) increases the stability of the thioredoxin from Escherichia coli.

ROSSI, MOSE';BARTOLUCCI, SIMONETTA
2001

Abstract

Glu85 in the Escherichia coli thioredoxin, which is localized in the loop between beta4 and beta5, was substituted with the Arg present in the corresponding position in Bacillus acidocaldarius thioredoxin. This suggested that it could play an important role in the structure and thermostability of this protein owing to its involvement in numerous interactions. The effects of the mutation on the biophysical properties were analysed by circular dichroism, spectrofluorimetry and limited proteolysis, supported by molecular dynamics data. As modelling predicted, an increase in stability for E85R due to additional H-bonds between the beta5 and alpha4 regions was observed.
2001
A single point mutation (Glu85Arg) increases the stability of the thioredoxin from Escherichia coli / Pedone, E.; Saviano, M.; Rossi, Mose'; Bartolucci, Simonetta. - In: PROTEIN ENGINEERING. - ISSN 0269-2139. - STAMPA. - 14:4(2001), pp. 255-260. [10.1093/protein/14.4.255]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/132270
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