The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cloned and expressed in simian CV1 and murine NIH3T3 cell lines. The recombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as the wild-type Ss beta-gal and has no cytotoxic effect on the host cells. Its possible use as a reporter gene is also proposed, and a comparison with other reporter gene systems is made.
Gene expression of a thermostable b-galactosidase in mammalian cells and its application in assays of eukariotic promoter activity / Cannio, R.; DE PASCALE, D.; Rossi, M.; Bartolucci, Simonetta. - In: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY. - ISSN 0885-4513. - STAMPA. - 19:Pt 2(1994), pp. 233-244.
Gene expression of a thermostable b-galactosidase in mammalian cells and its application in assays of eukariotic promoter activity.
BARTOLUCCI, SIMONETTA
1994
Abstract
The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cloned and expressed in simian CV1 and murine NIH3T3 cell lines. The recombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as the wild-type Ss beta-gal and has no cytotoxic effect on the host cells. Its possible use as a reporter gene is also proposed, and a comparison with other reporter gene systems is made.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
