In the seminal plasma of normal men a protein, immunologically related to a major protein (RSV-IV) secreted from the rat seminal vesicle epithelium, was detected by competition with RSV-IV in a specific radioimmunoassay using a rabbit anti RSV-IV antiserum. The protein was partly (80%) purified by column chromatography; characterization by gel electrophoresis indicated that the protein is slightly basic and has a molecular weight of 140 000. The protein was present on the surface of human spermatozoa as well as in the ejaculates of azoospermic men and it is believed to be a sperm-binding protein.
Identification and preliminary characterization of a sperm-binding protein in normal human semen / Abrescia, Paolo; Lombardi, Gaetano; M., DE ROSA; L., Quagliozzi; J., Guardiola; S., Metafora. - In: JOURNAL OF REPRODUCTION AND FERTILITY. - ISSN 0022-4251. - STAMPA. - 73:(1985), pp. 71-77.
Identification and preliminary characterization of a sperm-binding protein in normal human semen
ABRESCIA, PAOLO;LOMBARDI, GAETANO;
1985
Abstract
In the seminal plasma of normal men a protein, immunologically related to a major protein (RSV-IV) secreted from the rat seminal vesicle epithelium, was detected by competition with RSV-IV in a specific radioimmunoassay using a rabbit anti RSV-IV antiserum. The protein was partly (80%) purified by column chromatography; characterization by gel electrophoresis indicated that the protein is slightly basic and has a molecular weight of 140 000. The protein was present on the surface of human spermatozoa as well as in the ejaculates of azoospermic men and it is believed to be a sperm-binding protein.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
