Effects of the integration with saccharomyces cerevisiae on the metabolic profile in the Mediterranean Italian buffalo.

The integration in the diet with yeast culture of the saccharomyces cerevisiae, increases the performance of the buffalo cow during over the lactation, improving the digestibility of the diet. The aim of this work was to evaluate the effects of the integration of the diet with Saccharomyces cerevisiae on the metabolic profile of the bffalo cows. The trial was made using 16 buffalo cows with days in milk (DIM) of 85,4±37,4. The buffaloes were fed with a diet that had: 0,85 MUF/kg D.M., and a concentration of CP, NDF and NSC of the 14, 42 and 30%, respectively. The buffaloes were divided in two groups: group C =control; group T = integration with 98 milliards of FCU of a yeast culture of Saccharomyces cerevisiae (Super 6 – Vitasol). At the beginning, in the half and at the end of the trial, lasted 90 days, from the buffaloes were collected a sample of blood, before the feeding, from the jugular vein. Immediately after sampling the ammonia (NH3) was measured on whole blood with a rapid method (Ammonia kit, Menarini, Florence, Italy) that has a high correlation (r = 0.988) with the enzymatic UV-method. Samples were centrifuged at 3000 g for 15 min and serum was stored at -18°C until required for analyses. On the plasma was dosed the glycaemia, while on the serum was dosed the urea, creatinine, total cholesterol, HDL cholesterol, serum enzymes (ALT, AST, ALP, CK, LDH). The differences in the two groups among the levels of the haematic parameters were analysed by ANOVA for repeated measures. The integration of the diet with Saccharomyces cerevisiae had no effect on the haematic parameters. From the anlayses of the data was possible put in evidence in the buffaloes of the T group lower haematic values of glucose (P<0,08) and ammonia (P<0,08) than the C group (table 1). The higher recourse to the fat storage used to produce an higher quantity of milk in the buffaloes of the T group was responsible to go down the glucose and urea values in the blood. Therefore the animals of the T group produced, during all the trial, ~ 1.7 kg of ECM (P<0,01) and 26 grams of protein more than the C group. The lower levels of ammonia could show the consequence of the higher activity of the rumen micro flora (Dawson, 1993), that, in our case (Zicarelli et al. 2005), improved the organic matter digestibility. In conclusion we can say that, in the buffaloes of the T group, the higher availability of the energy thanks to the better digestibility of the organic matter has promoted the milk yield influencing animals metabolism.