Quantitative dtection of Secale cerale by real time PCR amplification

Wheat, barley, rye free diet is required to control coeliac disease and therefore adequate analytical tools are necessary to check gluten-free products. At the moment, official analytical methods are based on immunochemical assays for gliadin detection. However, DNA-based methods for detection of specific sequences have been extensively proposed as alternative to the protein-based ones, e.g. for transgenic contamination analysis. The aim of this work has been to develop an alternative quantification method for rye-content evaluation in raw materials and processed food based on real-time PCR approach using both SYBR green detection system and TaqMan fluorogenic probe