Mitochondria transplantation therapy is emerging as a novel therapeutic approach to promote neuroprotection in central nervous system (CNS) disorders. Here, we present a protocol for mitochondrial surface modification that enhances the restoration of intracellular adenosine triphosphate (ATP) levels under conditions of oxidative stress. We describe steps for isolating mitochondria, preparing the dual-tube system, and encapsulation with lipid coat. We then detail procedures for performing assays and analyses. For complete details on the use and execution of this protocol, please refer to Nakano et al. 1
Protocol for mitochondria lipid encapsulation using a dual-tube system for mitochondria transfer therapy / Hamanaka, G., Back, D.B., Licastro, E., Ishikane, S., Nakano, T., Hayakawa, K.. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 6:4(2025). [10.1016/j.xpro.2025.104174]
Protocol for mitochondria lipid encapsulation using a dual-tube system for mitochondria transfer therapy
Licastro E.;
2025
Abstract
Mitochondria transplantation therapy is emerging as a novel therapeutic approach to promote neuroprotection in central nervous system (CNS) disorders. Here, we present a protocol for mitochondrial surface modification that enhances the restoration of intracellular adenosine triphosphate (ATP) levels under conditions of oxidative stress. We describe steps for isolating mitochondria, preparing the dual-tube system, and encapsulation with lipid coat. We then detail procedures for performing assays and analyses. For complete details on the use and execution of this protocol, please refer to Nakano et al. 1I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
