Aneuploidy, an abnormal chromosome set, can ensue from failure of the spindle checkpoint, the safeguard mechanism that halts anaphase onset until mitotic spindle assembly. Inefficiency of cells to maintain the normal chromosome set across cell generations has been linked to tumorigenesis and senescence. Here we show that oxidative stress overrides the spindle checkpoint mechanism. Oxidant challenge of checkpoint-arrested cells led to proteolysis of the anaphase inhibitor securin and mitotic cyclins. This appeared consequent to loss of cyclin B-cdk1 activity caused by oxidant-induced reversal of cdk1 inhibitory phosphorylation. These observations may provide a link between aneuploidy occurrence and oxidative stress.

Oxidative stress overrides the spindle checkpoint / D'Angiolella, V; Santarpia, C; Grieco, Domenico. - In: CELL CYCLE. - ISSN 1538-4101. - STAMPA. - 6:(2007), pp. 576-579.

Oxidative stress overrides the spindle checkpoint

GRIECO, DOMENICO
2007

Abstract

Aneuploidy, an abnormal chromosome set, can ensue from failure of the spindle checkpoint, the safeguard mechanism that halts anaphase onset until mitotic spindle assembly. Inefficiency of cells to maintain the normal chromosome set across cell generations has been linked to tumorigenesis and senescence. Here we show that oxidative stress overrides the spindle checkpoint mechanism. Oxidant challenge of checkpoint-arrested cells led to proteolysis of the anaphase inhibitor securin and mitotic cyclins. This appeared consequent to loss of cyclin B-cdk1 activity caused by oxidant-induced reversal of cdk1 inhibitory phosphorylation. These observations may provide a link between aneuploidy occurrence and oxidative stress.
2007
Oxidative stress overrides the spindle checkpoint / D'Angiolella, V; Santarpia, C; Grieco, Domenico. - In: CELL CYCLE. - ISSN 1538-4101. - STAMPA. - 6:(2007), pp. 576-579.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/105092
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