Antibacterial effects of various arginine- and lysine-rich polycationic proteins and polymers were evaluated by broth and solid dilution assay on a range of foodborne pathogens, Gram-positive and Gram-negative bacteria. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of a-poly-L-lysine (poly-lys), a-poly-L-arginine (poly-arg) and protamines from herring sperm (clupeine sulphate) and salmon sperm (salmine sulphate) were determined on Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Shigella sonnei, Escherichia coli O157:H7 and Pseudomonas aeruginosa. All these molecules showed antibacterial activity on all strains with different MIC and MBC values. The molecular mechanisms underlying the effect of a-poly- L-arginine might be related to the entrance of the molecule into the cell. In fact a-poly-L-arginine labelled with 7-Diethylamino coumarin-3-carboxylic acid, succinimidyl ester (DEAC,SE) showed ability to permeate the cell membrane of B. cereus and E. coli O157:H7

Antimicrobial activity of various cationic molecules on foodborne pathogens / Conte, M; Aliberti, Francesco; Fucci, Laura; Piscopo, Marina. - In: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY. - ISSN 1573-0972. - STAMPA. - 23:(2007), pp. 1679-1683.

Antimicrobial activity of various cationic molecules on foodborne pathogens

ALIBERTI, FRANCESCO;FUCCI, LAURA;PISCOPO, MARINA
2007

Abstract

Antibacterial effects of various arginine- and lysine-rich polycationic proteins and polymers were evaluated by broth and solid dilution assay on a range of foodborne pathogens, Gram-positive and Gram-negative bacteria. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of a-poly-L-lysine (poly-lys), a-poly-L-arginine (poly-arg) and protamines from herring sperm (clupeine sulphate) and salmon sperm (salmine sulphate) were determined on Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Shigella sonnei, Escherichia coli O157:H7 and Pseudomonas aeruginosa. All these molecules showed antibacterial activity on all strains with different MIC and MBC values. The molecular mechanisms underlying the effect of a-poly- L-arginine might be related to the entrance of the molecule into the cell. In fact a-poly-L-arginine labelled with 7-Diethylamino coumarin-3-carboxylic acid, succinimidyl ester (DEAC,SE) showed ability to permeate the cell membrane of B. cereus and E. coli O157:H7
2007
Antimicrobial activity of various cationic molecules on foodborne pathogens / Conte, M; Aliberti, Francesco; Fucci, Laura; Piscopo, Marina. - In: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY. - ISSN 1573-0972. - STAMPA. - 23:(2007), pp. 1679-1683.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/104207
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